Q tof maxis impact 2
Manufactured by Bruker
Sourced in Germany
The Q-TOF Maxis Impact II is a high-resolution quadrupole time-of-flight (Q-TOF) mass spectrometer designed for accurate mass determination and high-sensitivity analysis. It features a robust impact ionization source, a high-performance quadrupole mass filter, and a reflectron time-of-flight analyzer. The instrument is capable of precise mass measurements and can be used for a variety of analytical applications.
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4 protocols using q tof maxis impact 2
1
High-Resolution Mass Spectrometry of Biomolecules
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(i) High-resolution electrospray ionization mass spectrometry analysis. Q-TOF Maxis Impact II (Bruker Daltonics) mass spectrometer with electrospray ionization was used for sample analysis. Lyophilized HPLC fractions from biochemical reactions were dissolved in 0.1% formic acid in deionized water and introduced directly to the instrument using the syringe pump. The spectra were recorded in a positive ion mode in a range from 100 m/z to 750 m/z. The temperature of ion source was 200°C, pressure of carrier gas 2.5 bar, the gas flow 5 mL/min, the voltage at the capillary 4 kV. The fragmentation spectra were recorded in AutoMS mode.
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2
Comprehensive Lipidomic Profiling of Sunflower
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Samples were processed using mass-spectrometry coupled with reversed-phase ultra-performance liquid chromatography (UPLC-MS) (ACQUITY UPLC System; Waters, USA) in positive and negative ionization modes in Q-TOF Maxis Impact II, Bruker Daltonik, Germany. Settings: Ion Polarity: positive/negative, Scan mode: MS, Mass range: 50 -1200 m/z, Spectra rate: 2 Hz.
UPLC separation was performed on the C8 Acquity Beh column (2.1 mm Х 100 mm, 1.7-μm particle size; Waters) and the Acquity BEH C8 1.7 μm Vanguard precolumn (Waters) at 60 °C.
The detailed information can be found in Methods S3 .
Previously we have validated the extraction and profiling technique for FAs [56 (link)] and TAGs [57 ] in sunflower.
UPLC separation was performed on the C8 Acquity Beh column (2.1 mm Х 100 mm, 1.7-μm particle size; Waters) and the Acquity BEH C8 1.7 μm Vanguard precolumn (Waters) at 60 °C.
The detailed information can be found in Methods S
Previously we have validated the extraction and profiling technique for FAs [56 (link)] and TAGs [57 ] in sunflower.
3
Sunflower Lipid Profiling by UPLC-MS
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Samples were processed using mass spectrometry (UPLC-MS) coupled with reversed-phase ultraperformance liquid chromatography (ACQUITY UPLC System; Waters, USA) in positive and negative ionization modes in Q-TOF Maxis Impact II, Bruker Daltonik, Germany. Settings: Ion Polarity: positive/negative, Scan mode: MS, Mass range: 50 -1200m/z, Spectra rate: 2 Hz.
UPLC separation was performed on the C8 Acquity Beh column (2.1 mm Х 100 mm, 1.7-µm particle size; Waters) and the Acquity BEH C8 1.7 µm Vanguard precolumn (Waters) at 60 °C.
The detailed information can be found in Methods S3.
Previously we have validated the extraction and pro ling technique for Sun ower FAs [54] and TAGs [55] .
UPLC separation was performed on the C8 Acquity Beh column (2.1 mm Х 100 mm, 1.7-µm particle size; Waters) and the Acquity BEH C8 1.7 µm Vanguard precolumn (Waters) at 60 °C.
The detailed information can be found in Methods S3.
Previously we have validated the extraction and pro ling technique for Sun ower FAs [54] and TAGs [55] .
4
High-Resolution Mass Spectrometry of Biomolecules
Check if the same lab product or an alternative is used in the 5 most similar protocols
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High resolution electrospray ionization mass spectrometry (ESI-MS) analysis. Q-TOF Maxis Impact II (Bruker Daltonics) mass-spectrometer with electrospray ionization was used for sample analysis. Lyophilized HPLC fractions from biochemical reactions were dissolved in 0.1% formic acid in deionized water and introduced directly to the instrument using the syringe pump.
The spectra were recorded in a positive ion mode in a range from 100 m/z to 750 m/z. The temperature of ion source was 200 ºC, pressure of carrier gas 2.5 bar, the gas flow 5 ml/min, the voltage at the capillary 4 kV. The fragmentation spectra were recorded in AutoMS mode.
Matrix Assisted Laser Desorption Time of Flight (MALDI-TOF) mass spectrometry.
Sample aliquots were combined with the matrix mix (Sigma-Aldrich) on a steel target. The mass spectra were recorded on an UltrafleXtreme MALDI-TOF/TOF mass spectrometer (Bruker Daltonics) equipped with a neodymium laser. The molecular MH+ ions were measured in reflector mode; the accuracy of the measured results was within 0.1 Da.
The spectra were recorded in a positive ion mode in a range from 100 m/z to 750 m/z. The temperature of ion source was 200 ºC, pressure of carrier gas 2.5 bar, the gas flow 5 ml/min, the voltage at the capillary 4 kV. The fragmentation spectra were recorded in AutoMS mode.
Matrix Assisted Laser Desorption Time of Flight (MALDI-TOF) mass spectrometry.
Sample aliquots were combined with the matrix mix (Sigma-Aldrich) on a steel target. The mass spectra were recorded on an UltrafleXtreme MALDI-TOF/TOF mass spectrometer (Bruker Daltonics) equipped with a neodymium laser. The molecular MH+ ions were measured in reflector mode; the accuracy of the measured results was within 0.1 Da.
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