Methylcellulose containing medium

Manufactured by STEMCELL

Methylcellulose-containing medium is a laboratory reagent used in various cell culture applications. It serves as a viscosity-enhancing agent, supporting the suspension and growth of cells in a semi-solid or gel-like environment.

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Lab products found in correlation

Myelocult, by STEMCELL (1 mentions)

2 protocols using methylcellulose containing medium

CML CD34+ Cell Colony Assay

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Primary CML CD34+ cells were cultured for 3 days with 100 nM Meds433 or control and then 500 cells/dish were suspended in IMDM supplemented with 2% FBS. Cells were plated in methylcellulose-containing medium (StemCell Technologies) in 35-mm dishes based on the manufacturer’s instruction and colonies were scored and counted after 15 days.

Houshmand M., Vitale N., Orso F., Cignetti A., Molineris I., Gaidano V., Sainas S., Giorgis M., Boschi D., Fava C., Passoni A., Gai M., Geuna M., Sora F., Iurlo A., Abruzzese E., Breccia M., Mulas O., Caocci G., Castagnetti F., Taverna D., Oliviero S., Pane F., Lolli M.L., Circosta P, & Saglio G. (2022). Dihydroorotate dehydrogenase inhibition reveals metabolic vulnerability in chronic myeloid leukemia. Cell Death & Disease, 13(6), 576.

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CML Patient BMMC Hematopoietic Assay

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BMMCs from CML patients or normal CD34+ PBMCs (1 × 10⁶/mL) were drug-treated for 48 hr in low oxygen, resuspended in Myelocult (no. 05100, Stem Cell Technology, Vancouver, BC, Canada) supplemented with hydrocortisone (10⁻⁶ M), seeded (BMMCs from CML patients 2 × 10⁶/35-mm dish; normal CD34+ PBMC 5 × 10³/35-mm dish) onto a feeder layer of irradiated (8,000 cGy) murine stromal cells (M2-10B4 cell line) and incubated in normoxia. Half medium was replaced weekly. After 5 weeks, cells were recovered, counted, and replated (5 × 10⁴ cells/35-mm dish) in methylcellulose-containing medium (no. 04435, Stem Cell Technology) (Sutherland et al., 1989 (link)). Colonies were scored after 14 days of incubation.

Tusa I., Cheloni G., Poteti M., Gozzini A., DeSouza N.H., Shan Y., Deng X., Gray N.S., Li S., Rovida E, & Dello Sbarba P. (2018). Targeting the Extracellular Signal-Regulated Kinase 5 Pathway to Suppress Human Chronic Myeloid Leukemia Stem Cells. Stem Cell Reports, 11(4), 929-943.

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