Log In Sign up
The largest database of trusted experimental protocols

Bouin solution

Manufactured by Fujifilm
Visit Supplier

Bouin solution is a fixative used in histological and cytological laboratory procedures. It is a mixture of picric acid, formaldehyde, and acetic acid. The primary function of Bouin solution is to preserve and harden tissue samples, enabling effective sectioning and staining for microscopic examination.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Bouin’s solution (7 citations)

2 protocols using bouin solution

1

Histological Analysis of Testes and Ovaries

Check if the same lab product or an alternative is used in the 5 most similar protocols
For histology of testes, samples were fix in one ml of Bouin solution (Wako 023-17361) overnight and in 10% buffered formaldehyde overnight. For histology of ovaries, samples were fixed in one ml of 10% buffered formaldehyde overnight. After fixation, samples were embedded, sectioned and stained with hematoxylin–eosin. Stained samples were analyzed by NHSL (New Histo Science Laboratory).
Matsuzaki K., Kondo S., Ishikawa T, & Shinohara A. (2019). Human RAD51 paralogue SWSAP1 fosters RAD51 filament by regulating the anti-recombinase FIGNL1 AAA+ ATPase. Nature Communications, 10, 1407.
+ Open protocol
+ Expand
2

3D Imaging of Mouse Skulls and Embryos

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse skulls and embryos were scanned using a micro-CT scanner (ScanXmate-E090S, Comscantecno Co., Ltd., Kanagawa, Japan). For skull analysis, the head region of 22-week-old male mice were scanned at a tube voltage of 80 kV and a tube current of 50 μA. We used TRI/3D-BON (RATOC Systems, Osaka, Japan) and Image J software (NIH) for reconstructing the three-dimensional images of skulls and measuring the linear distances between landmarks, respectively. Before embryo scanning, E15.5 mouse embryos were fixed with Bouin solution (Wako) at room temperature, stored in 70% ethanol and then treated with 1% phosphotungstic acid. These samples were scanned at a tube voltage of 40 kV and a tube current of 100 μA. Section images were analyzed using the OsiriX (www.osirix-viewer.com) software.
Matsumura K., Seiriki K., Okada S., Nagase M., Ayabe S., Yamada I., Furuse T., Shibuya H., Yasuda Y., Yamamori H., Fujimoto M., Nagayasu K., Yamamoto K., Kitagawa K., Miura H., Gotoda-Nishimura N., Igarashi H., Hayashida M., Baba M., Kondo M., Hasebe S., Ueshima K., Kasai A., Ago Y., Hayata-Takano A., Shintani N., Iguchi T., Sato M., Yamaguchi S., Tamura M., Wakana S., Yoshiki A., Watabe A.M., Okano H., Takuma K., Hashimoto R., Hashimoto H, & Nakazawa T. (2020). Pathogenic POGZ mutation causes impaired cortical development and reversible autism-like phenotypes. Nature Communications, 11, 859.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!