Anti mhc 2 pe cy7

Manufactured by Thermo Fisher Scientific

The Anti-MHC II-PE-CY7 is a fluorescently-labeled antibody that binds to major histocompatibility complex class II (MHC II) molecules. It is designed for use in flow cytometry applications to identify and quantify MHC II-expressing cells.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using anti mhc 2 pe cy7

Multiparametric Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following fluorochrome-labelled monoclonal antibodies and staining reagents were used according to manufacturer’s protocols: lamina propria cells were stained with anti-CD11c-APC, anti-CD64-APC (eBioscience), anti-CD11b-PerCP Cy5.5 (Biolegend), anti-CD103-PE/Horizon 421(BD Pharmingen), anti-MHC II-PE-CY7, anti-CD24- PE-CY7 (eBioscience), anti-CD45.2-FITC (eBioscience), anti-CD45.1-Pacific blue (Biolegend) and 4′,6-diamidino-2-phenylindole. TH17 and TH1 cells were stained with anti-IL-17A-PE, anti-CD3-biotin, SA-PerCP, anti-CD4-APC, anti-CD8-FITC, anti-CD45-Pacific Blue anti-IFNγ-PE-CY7 and anti-CD121A-PE. The cells were analysed with LSR Fortessa flow cytometer (BD) or sorted with a FACSAria machine (BD). Flow cytometry analysis was done with the FlowJo software.

Aychek T., Mildner A., Yona S., Kim K.W., Lampl N., Reich-Zeliger S., Boon L., Yogev N., Waisman A., Cua D.J, & Jung S. (2015). IL-23-mediated mononuclear phagocyte crosstalk protects mice from Citrobacter rodentium-induced colon immunopathology. Nature Communications, 6, 6525.

+ Open protocol

+ Expand

Flow Cytometry Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies for flow cytometry were purchased from eBioscience company as follows: anti-CD45-FITC (11-0451); anti-F4/80-APC (17-4801); anti-MHCII-PE-Cy7 (25-5321); anti-CD11B-PE (12-0112); anti-Ly6G-PercpCy5.5 (45-5931); anti-EpCAM-APC (17-5791); anti-CD24-PE (12-0241); anti-Sca-1-Percp-Cy5.5 (45-5981);anti-CD4-APC (17-0042); anti-Tcrb-PercpCy5.5 (45-5961); anti-IL-17A-PE (12-7178). Cell suspensions were prepared by sieving and gentle pipetting. Cells were washed in ice-cold FACS buffer (2% FCS, 2 mM EDTA in PBS), then incubated with each antibody for 30 min and washed twice with FACS buffer. Data were acquired on a Beckman Gallios flow cytometer and cells were sorted by Beckman Moflo Astrios. Flowjo software was used for data analysis.

Yang D., Chen X., Wang J., Lou Q., Lou Y., Li L., Wang H., Chen J., Wu M., Song X, & Qian Y. (2019). Dysregulated Lung Commensal Bacteria Drive Interleukin-17B Production to Promote Pulmonary Fibrosis through Their Outer Membrane Vesicles. Immunity, 50(3).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!