Biomass was harvested for DNA extraction via vacuum filtration using sterile 0.22-μm membrane disc filters (Millipore, Billerica, MA, USA) or by centrifugation at 12,000 rpm for 30 min. Extraction was performed using the Fast DNA Spin Kit for Soils (MP Biomedicals, USA), according to the manufacturer's instructions. DNA was stored at −20°C prior to subsequent analysis.
0.22 μm membrane disc filters
Manufactured by Merck Group
Sourced in United States
The 0.22-μm membrane disc filters are a type of lab equipment used for filtration purposes. They feature a pore size of 0.22 micrometers and are designed to remove particulates, microorganisms, and other contaminants from liquids or gases passing through them.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 0.22 μm membrane disc filters
1
DNA Extraction from Biomass
2
Biosolids DNA Extraction and Quantification
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DNA was extracted from vacuum-filtered biosolids collected using sterile 0.22-μm membrane disc filters (Millipore, Billerica, MA, USA) or by pelleting via centrifugation at 12,000 rpm for 30 min. Extraction was performed using the Fast DNA Spin Kit for Soils (MP Biomedicals, USA) according to the manufacturer's instructions. DNA was stored at −20 °C prior to subsequent analysis. It was not possible to perform the HT-qPCR for ARGs in samples from the river upstream the WWTP due to low DNA concentration.
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