The α-ketol (Me) preparations were purified firstly by RP-HPLC on Macherey–Nagel Nucleosil 5 ODS column (250 × 4 mm, 5 μm) using the solvent mixture methanol–water (linear gradient from 76:24 to 96:4, by volume) at a flow rate of 0.4 mL/min. Final purification was carried out by NP-HPLC on Macherey–Nagel Nucleodur 100–3 column (250 × 4.6 mm, 3 μm) eluted with hexane/isopropanol (99:1, by volume), flow rate 0.4 mL/min. Enantiomers of purified α-ketol methyl ester were separated on Chiralcel OB-H column (250 × 0.46 mm, 5 μm) with hexane/isopropanol 94:6 (by volume), flow rate 0.4 mL/min.
Nucleodur 100 3 column
Manufactured by Macherey-Nagel
Nucleodur 100–3 is a reversed-phase high-performance liquid chromatography (HPLC) column. It is designed for the separation and analysis of a variety of organic compounds. The column features a porous silica-based stationary phase with a particle size of 3 μm, which provides efficient and high-resolution separations.
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Lab products found in correlation
3 protocols using nucleodur 100 3 column
1
Purification and Separation of α-Ketol Methyl Esters
2
GC-MS Analysis of Methyl Esters
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The methyl esters of the products (or their methyl ester TMS derivatives) were subjected to direct GC–MS analysis after amino cartridge purification and derivatization. Alternatively, the methyl esters of the products were preliminarily separated by NP‐HPLC. Products were separated as methyl esters by NP‐HPLC on a Macherey–Nagel Nucleodur 100‐3 column (250×4.6 mm, 3 μm) by using a mixture of hexane/2‐propanol (98:2, v/v) at a flow rate of 0.4 mL min−1. Products were collected and rechromatographed by NP‐HPLC, eluting with hexane/2‐propanol (99.8:0.2, v/v) at a flow rate of 0.4 mL min−1.
3
Purification and Separation of Chiral Compounds
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The products were purified first using RP-HPLC on a Macherey–Nagel Nucleosil 5 ODS column (250 × 4 mm, 5 µm) using the solvent mixture methanol/water (linear gradient from 76:24 to 96:4, by volume) at a flow rate of 0.4 mL/min. The peaks of the products were collected and purified using NP-HPLC on a Macherey–Nagel Nucleodur 100–3 column (250 × 4.6 mm, 3 μm) using the solvent mixture hexane/isopropanol (98.5:1.5, by volume) at a flow rate of 0.4 mL/min. After NaBH4 reduction, the final purification of the products was performed using NP-HPLC as described above. Product enantiomers (methyl esters) were separated on a Chiralcel OD-H column (250 × 4.6 mm, 5 µm) with hexane/isopropanol (97:3, by volume) at a flow rate of 0.4 mL/min.
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