Tissue sections were deparaffinized, activated at 95 °C in Dako target retrieval agent (pH 9, DakoCytomation, Glostrup, Denmark) for 30 min, and pretreated with 3% H2O2 for 5 min. An anti-SLAMF8 antibody (×200, rabbit polyclonal, bs-2473R; Bioss, Woburn, MA, USA), an anti-ALK antibody (mouse monoclonal antibody, 5A4, Leica Microsystems, Wetzlar, Germany), or an anti-CD30 antibody mouse monoclonal antibody (Ber-H2, Roche Diagnostics, Mannheim, Germany) was added and incubated at room temperature for 1 h. For staining, the EnVision kit was used according to the manufacturer’s instructions (HRP/DAB; DAKO). We defined positivity as when more than 50% of tumor cells were stained in their membranes and cytoplasms.
Hrpdab
Manufactured by Agilent Technologies
Sourced in Germany
The HRPDAB is a high-resolution pulsed doppler anemometer that measures air velocity and turbulence. It uses a high-frequency pulsed laser to detect the doppler shift of reflected light, providing precise measurements of air flow.
Automatically generated - may contain errors
3 protocols using hrpdab
1
Immunohistochemical staining of SLAMF8, ALK, and CD30
2
Immunohistochemistry of Formalin-fixed Tissues
3
Immunohistochemistry of Formalin-fixed Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Formalin-fixed, paraffin-embedded tissues were cut into 4μm sections. The sections were de-thawed in Xylene and rehydrated in sequentially diluted ethanol baths. Citric acid (s1399, Dako, USA) was used for antigen retrieval and 3% H2O2 was used to block endogenous peroxidase activity. Sections were then incubated O/N at 4°C with primary antibody and 1 h at RT with secondary antibody. Immunohistochemistry was carried out using Streptavidin (Life technologies #SNN1004), Envision + System (Dako), and HRPDAB (Dako) colorimetric detection. Antibody details in Key Resources Table .
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