All reagents used were of analytical grade. Dulbecco’s phosphate-buffered saline (PBS), Minimum Essential Medium (MEM) and trypsin-EDTA were purchased from Corning (Glendale, AZ, United States of America). Lympholyte® was obtained from Cerdelane (Burlington, NC, United States of America). Hydrochloric acid (HCl) was purchased from Thermo Fisher Scientific (Waltham, MA, United States of America). Acridine Orange (AO), 4′,6-diamidino-2-phenylindole (DAPI), ethidium bromide, low-melting point agarose (LMPA), normal-melting point agarose (NMPA), tris(hydroxymethyl)aminomethane (Tris) and Triton X-100 were obtained from Sigma-Aldrich Srl (Milan, Italy). Ethylenediaminetetracetic acid disodium (Na2EDTA) and tetrasodium (Na4EDTA) salt, sodium chloride (NaCl) and sodium hydroxide (NaOH) were purchased from Carlo Erba Reagenti (Milan, Italy). Dimethyl sulfoxide (DMSO) and ethanol absolute were purchased from AppliChem GmbH (Darmstadt, Germany).
Acridine orange 4 6 diamidino 2 phenylindole dapi
Manufactured by Merck Group
Sourced in Angola
Acridine Orange (AO) and 4′,6-diamidino-2-phenylindole (DAPI) are fluorescent dyes commonly used in laboratory settings. AO is a nucleic acid-binding dye that emits green fluorescence when bound to DNA and orange fluorescence when bound to RNA. DAPI is a DNA-specific dye that produces a blue fluorescence when bound to DNA. Both dyes are widely used in various applications, such as cell staining, fluorescence microscopy, and flow cytometry, to visualize and study genetic material.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Merck Group (2 mentions)
Trypsin edta, by Corning (1 mentions)
Tris hydroxymethyl aminomethane tris, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions)
Low melting point agarose, by Merck Group (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by AppliChem (1 mentions)
Sodium hydroxide (naoh), by Carlo Erba (1 mentions)
Sodium chloride (nacl), by Carlo Erba (1 mentions)
Ethanol absolute, by AppliChem (1 mentions)
Rpmi 1640 media, by Thermo Fisher Scientific (1 mentions)
2 7 dichlorofluorescein diacetate dcf da, by Merck Group (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Sodium dihydrogen phosphate, by Merck Group (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Tween 20, by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Minimal essential medium, by Thermo Fisher Scientific (1 mentions)
2 protocols using acridine orange 4 6 diamidino 2 phenylindole dapi
1
Fluorescent Staining Techniques for Cell Analysis
2
Niclosamide Compound Characterization
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Niclosamide was purchased from Santa Cruz Biotechnology, Inc. (Texas, U.S.A.). The powder was dissolved in dimethyl sulfoxide (DMSO) and stored as aliquots (20 mM) at -80 ºC. Roswell Park Memorial Institute-1640 (RPMI-1640) media, Minimal essential medium (MEM), Fetal bovine serum, Penicillin, Streptomycin, Trypsin/EDTA were purchased from Invitrogen Life Technologies, USA. Acridine orange (AO), 4′,6-diamidino-2-phenylindole (DAPI), Acrylamide, Ammonium acetate, 2',7'-dichlorofluorescein diacetate (DCFDA), Dithiotheritol, Dihydrogen sodium phosphate, EGTA, tetraethylbenzimidazolyl carbocyanine iodide (JC-1), Methylthiazolyldiphenyl-tetrazolium bromide (MTT), Nonidet P-40, N1, N1-Dimethyl bis Acrylamide, NADH, Propidium Iodide, Proteinase K, RNAse A, Sodium pyrophosphate, Tween 20, Triton-X-100 and all other reagents unless mentioned were obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Primary antibodies included poly (ADP-ribose) polymerase (PARP) (116/89 kDa), NF-κB (P65) (65 kDa), Bcl-2 (28 kDa), Bax (20 kDa), Cytochrome C (14 kDa), p53 (53 kDa), total Akt (60 kDa), Ser 473 phosphorylated Akt (p-Akt) (60 kDa), p-4E-BP1 (21 kDa), p-70S6K1 (70 kDa) and Actin (42 kDa) . Horseradish peroxidase-conjugated secondary anti-mouse and anti-rabbit antibodies were purchased from Cell Signaling Technology, Danvers, MA, USA.
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