The anti-SK was kindly provided by the Dolph lab (Abou Tayoun et al., 2011 (link)). The anti-SK antibody was used at a concentration of 1:2000, mouse anti-V5 antibody (Thermo Fisher Scientific) was used at a concentration of 1:200, anti-GFP antibody was used at a concentration of 1:250 (Thermo Fisher Scientific), and an anti-HRP antibody was used at concentration of 1:100 (Jackson ImmunoResearch Lab). All secondary antibodies were used at a concentration of 1:600 or 1:1000 (Alexa Flours from Thermo Fisher Scientific). Detailed staining protocols are available upon request.
Anti hrp antibody
Manufactured by Jackson ImmunoResearch
An anti-HRP (horseradish peroxidase) antibody is a laboratory reagent used in various immunoassay techniques. It is a specific antibody that binds to the HRP enzyme, which is commonly used as a reporter or detection label in applications such as ELISA, Western blotting, and immunohistochemistry. The primary function of the anti-HRP antibody is to provide a means of detecting and quantifying the presence of the HRP enzyme in these assays.
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2 protocols using anti hrp antibody
1
Antibody Staining Protocol for Protein Detection
2
Antibody Staining Protocol for Protein Detection
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The anti-SK was kindly provided by the Dolph lab (Abou Tayoun et al., 2011 (link)). The anti-SK antibody was used at a concentration of 1:2000, mouse anti-V5 antibody (Thermo Fisher Scientific) was used at a concentration of 1:200, anti-GFP antibody was used at a concentration of 1:250 (Thermo Fisher Scientific), and an anti-HRP antibody was used at concentration of 1:100 (Jackson ImmunoResearch Lab). All secondary antibodies were used at a concentration of 1:600 or 1:1000 (Alexa Flours from Thermo Fisher Scientific). Detailed staining protocols are available upon request.
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