TAM and 18α-GA were provided by Sigma-Aldrich (St. Louis, MO, USA). TGF-β1 was obtained from Pepro Tech (Rocky Hill, NJ, USA). MS-275 was supplied by MedChemExpress (Monmouth Junction, NJ, USA). Antibodies against Cx43 and α-SMA were obtained from Abcam (Cambridge Science Park, Cambridge, England). E-ca, N-ca, p-Akt, Akt, c-Src and p-c-Src antibodies and LY294002 (PI3K inhibitor) were purchased from Cell Signaling Technology (Danvers, MA, USA). β-actin and GAPDH antibodies were purchased from Bioworld Technology, Co, Ltd. (Nanjing, China). Dasatinib was from Aladdin Bio-Chem Technology Co.LTD (Shanghai, China). IGF-1 was from PeproTech, Inc. (Rocky Hill, USA). Other reagents were all provided from Sigma-Aldrich unless otherwise noted.
Ms 275
Manufactured by MedChemExpress
Sourced in United States, China
MS-275 is a histone deacetylase (HDAC) inhibitor. It inhibits HDAC1, HDAC3, and HDAC8 enzymes. The product is intended for laboratory research use only.
Automatically generated - may contain errors
Lab products found in correlation
Ly294002, by Cell Signaling Technology (1 mentions)
C src, by Cell Signaling Technology (1 mentions)
Igf 1, by Thermo Fisher Scientific (1 mentions)
P c src, by Cell Signaling Technology (1 mentions)
18α ga, by Merck Group (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
Tgf β1, by Thermo Fisher Scientific (1 mentions)
Gapdh, by Bioworld Technology (1 mentions)
β actin, by Bioworld Technology (1 mentions)
Collagenase 1, by Yeasen (1 mentions)
Recombinant mouse m csf, by Novoprotein (1 mentions)
2 protocols using ms 275
1
Epithelial-Mesenchymal Transition Regulation
2
Isolation and Stimulation of Pancreatic, Liver, and Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Pancreatic leukocytes were isolated using the collagenase digestion method described for flow cytometry analysis.32 Liver cells and spleen cells were acquired by simply smashing tissues and then washing with FACS buffer (HBSS + 2% NCS) for subsequent staining. Pancreatic acinar cells were isolated using collagenase I (0.2 mg/ml; Yeasen Biotech) and IV (0.2 mg/ml; Yeasen Biotech), shaken at 37°C for 10 min, and then dissociated with tips. Acinar cells were stimulated with caerulein (1 × 10−7 M) for 5 h before conditioned medium collection. Bone marrow cells were cultured with recombinant mouse M-CSF (50 ng/ml; Novoprotein, Suzhou, China) to generate BMDMs as previously described.33 (link) On day 6 of culturing, BMDMs were stimulated with LPS (100 ng/ml) for 15 min or 4 h, or injured acinar cell culture medium for further assays. βOHB (10 mM; Sigma-Aldrich), HDAC pan-inhibitor TSA (1 μM; MedChem Express, Shanghai, China) or HDAC1/3 inhibitor MS-275 (20 μM; MedChem Express) was administered 1 h before LPS stimulation.
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