All invasion assays were performed using Boyden chambers with 8 μm pores (Costar, Tewksbury, MA, USA) and all cells were starved overnight in 1% fetal bovine serum. For invasion, 2.5 × 104 cells in 0.1% bovine serum albumin were placed in the top chamber and allowed to migrate towards NIH-3T3-conditioned media in the lower chamber. For invasion, type I rat tail collagen (BD Biosciences, San Jose, CA, USA) was set using Boyden membrane and cells were allowed to invade for 22 h. After 22 h, cells were removed from the upper chamber and membranes were stained using the Hema3 system; the invasion assays were quantified by counting the number of cells as previously described.53 (link)
Boyden chambers with 8 μm pores
Manufactured by Corning
Sourced in United States
Boyden chambers with 8 μm pores are a laboratory equipment used for studying cell migration and invasion. These chambers consist of an upper and lower compartment separated by a porous membrane, typically with 8 μm pores. Cells are placed in the upper compartment and their ability to migrate through the pores to the lower compartment is measured, providing insights into their migratory and invasive properties.
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Lab products found in correlation
2 protocols using boyden chambers with 8 μm pores
1
Boyden Chamber Invasion Assay
2
Wound Healing and Cell Migration Assay
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Approximately 5 × 105 cells per well were seeded into 6-well plates and five parallel scratches or “wounds” (wide approximately equal to 500 μm) were marked after the cells becoming adherent. Migration of cells into the “wounds” was observed and images of areas flanking the intersections of the “wound” and the marked lines were taken at regular intervals over the course of 24 h. For migration assay, cells were harvested and resuspended in serum-free RPMI-1640 medium, and 5 × 104 cells were placed into 6.5-mm Boyden chambers with 8-μm pores (Corning Costar, Corning, NY, USA) and then inserted into the wells of a 24-well plate and incubated for 24 h in RPMI-1640 medium with 10% FBS prior to examination. Cells adhering to the lower surface were fixed and stained in a dye solution containing 0.05% crystal violet and counted under the microscope to determine their relative numbers. For each experiment, the number of cells in at least five random field on the underside of the filter was counted, and three independent filters were analyzed.
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