Alexa fluor 647 anti mouse cd206

Collateral vessels and the surrounding adipose tissue was harvested three days post-surgery and stained for α-smooth muscle actin FITC (1:200, clone 1A4, Sigma, St. Louis, MO), and Alexa Fluor 647 anti-mouse CD206 (1:200, clone C068C2, BioLegend, San Diego, CA) as previously described^{43 (link)}. Vessel diameters were quantified for the feeding vessel, the first branch, and the second branch, and CD206⁺ cells were quantified as previously described^{43 (link)}. Data presented include vessel diameter measurements of the feeding collateral vessel, the first branch of the collateral vessel, and the second branch of the collateral vessel. Three mice were repeated in three mice (n = 3).

Epinephrine, norEpinephrine, ICI 118,551 (all from Sigma, St. Louis, MO, USA), 2’5’-dideoxyadenosine (2’5’-DDA; Santa Cruz Biotech, Santa Cruz, CA, USA), SQ22536 (Santa Cruz Biotech), PKA inhibitor 14–22 amide (EMD Millipore, Billerica, MA, USA), and wortmannin (Santa Cruz Biotech) were all used at the concentrations listed for each figure. LPS (E. coli o111:B4) was purchased from Sigma. α₁ (prazosin, Sigma), α₂ (RX821002, Tocris, Bristol, United Kingdom), β₁ (betaxolol, Sigma), and β₃ (SR59230A, Sigma) adrenergic receptor antagonists were also used in these studies. Optimal concentrations for these reagents were determined in preliminary experiments (data not shown). AlexaFluor⁶⁴⁷ anti-mouse CD206 was from BioLegend (San Diego, CA, USA).