Ab58881

Manufactured by Abcam

Ab58881 is a monoclonal antibody that recognizes human CD4. CD4 is a glycoprotein that serves as a co-receptor for the T cell receptor, and is expressed on the surface of T helper cells, regulatory T cells, and monocytes.

Automatically generated - may contain errors

Lab products found in correlation

Ab22553, by Abcam (1 mentions) Ab5176, by Abcam (1 mentions) Ab2175, by Abcam (1 mentions) Ab70369, by Abcam (1 mentions) Ab32094, by Abcam (1 mentions) Ab26350, by Abcam (1 mentions) Nb100 690, by Novus Biologicals (1 mentions) C digit blot scanner, by LI COR (1 mentions) Ab14387, by Abcam (1 mentions) Ab96488, by Abcam (1 mentions) Nb100 182, by Novus Biologicals (1 mentions)

2 protocols using ab58881

Immunoblotting of DNA Damage Markers

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Primary antibodies against γH2AX (ab26350, 1:1000), pH3 Ser10 antibody (ab5176, 1:1000), pKAP1 (ab70369, 1000), pCDK Y15 (ab275958, 1:1000), RPA (ab2175, 1:1000), KAP1 (ab22553, 1:1000), CDK1 (ab32094, 1:1000), and FBH1 (ab58881, 1:100) were from Abcam. BrdU antibodies (recognizes IdU) was from BD biosciences (347580, 1:50). pRPA S4/8 antibodies (A300-245A, 1:1000) was from Fortis Life Sciences. Tubulin antibodies was from Novus Biologicals (NB100-690, 1: 1000).

Jennings L., Walters H.A, & Mason J.M. (2023). FBH1 deficiency sensitizes cells to WEE1 inhibition by promoting mitotic catastrophe. bioRxiv.

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Protein Expression Analysis by Western Blot

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Western blotting was performed using standard methods. Anti-DNA2 (1:500; ab96488), anti-MUS81 (1:1000, ab14387), and anti-FBH1 (1:100, ab58881) were from Abcam. Anti-FANCD2 (1:1000, NB100-182) was from Novus Biologicals. Proteins were detected using a C-DIGIT blot scanner (Licor). All uncropped blots are included in the source data file.

Mason J.M., Chan Y.L., Weichselbaum R.W, & Bishop D.K. (2019). Non-enzymatic roles of human RAD51 at stalled replication forks. Nature Communications, 10, 4410.

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