Model 2100

Manufactured by Agilent Technologies

The Agilent Model 2100 is a versatile laboratory instrument designed for various analytical applications. It serves as a platform for performing sample analysis and data processing. The Model 2100 is capable of generating and analyzing data, but a detailed description of its intended use or specific applications is not available.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (2 mentions) Rneasy kit, by Qiagen (1 mentions) Transcriptome analysis console 4, by Thermo Fisher Scientific (1 mentions) Clariom s assay for humans, by Thermo Fisher Scientific (1 mentions) Glass bead, by Merck Group (1 mentions) Qiashredder column, by Qiagen (1 mentions) Nanodrop, by Thermo Fisher Scientific (1 mentions) Humanht 12 v4 beadchip, by Illumina (1 mentions) Hiscansq system, by Illumina (1 mentions) Genomestudio gene expression module, by Illumina (1 mentions) Gene expression beadchip array, by Illumina (1 mentions) Model nd1000, by Thermo Fisher Scientific (1 mentions) Genelute mammalian total rna kit, by Merck Group (1 mentions) Sureselect human all exon v6 kit, by Agilent Technologies (1 mentions)

Spelling variants of this product

Agilent Model 2100 Bioanalyzer (41 citations) Model 2100 Bioanalyzer (34 citations) Model 2100 Total RNA BioAnalyzer (3 citations)

6 protocols using model 2100

Microarray Analysis of Human Transcriptome

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RNA was extracted with the RNeasy Kit (QIAGEN), and the RNA quality and quantity were assessed using a bioanalyzer (model 2100, Agilent Technologies). Microarray analysis was performed using the Clariom S Assay for humans (Thermo Fisher Scientific). The accession number was referred later. Data were analyzed by Transcriptome Analysis Console 4.0 (Thermo Fisher Scientific). Gene ontology (GO) and pathway analyses were performed using DAVID 6.8 Bioinformatics Resources (https://david.ncifcrf.gov). The data shown in this publication have been deposited in the NCBI Gene Expression Omnibus database (http://www.ncbi.nlm.nih.gov/geo/). The accession number of our microarray analysis data was GSE116820.

Ishii T., Ishikawa M., Fujimori K., Maeda T., Kushima I., Arioka Y., Mori D., Nakatake Y., Yamagata B., Nio S., Kato T.A., Yang N., Wernig M., Kanba S., Mimura M., Ozaki N, & Okano H. (2019). In Vitro Modeling of the Bipolar Disorder and Schizophrenia Using Patient-Derived Induced Pluripotent Stem Cells with Copy Number Variations of PCDH15 and RELN. eNeuro, 6(5), ENEURO.0403-18.2019.

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Yeast Total RNA Extraction and Sequencing

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Budding yeast cells (2 ODs) were harvested from log‐phase cultures growing in YPD at 25°C. Cells were lysed mechanically using glass beads (425–600 μm, Sigma‐Aldrich) in Precellys 24 Tissue Homogenizer. Samples were cleared on QIAshredder columns (Qiagen) and RNA was extracted using a RNeasy Mini Kit (Qiagen) following manufacturer’s instructions. Sample quality was checked first by their absorbance values (A260/A280 ≈ 2.2 and A260/A230 > 1.8), then by using a Bioanalyzer (Model 2100, Agilent) (RNA Integrity Number > 8). Library preparation (for Stranded mRNA‐seq/standard quantity, Ligation) and sequencing (Hiseq 4000 sequencing 1 × 50 bases) was performed by the GenomEast Platform (http://genomeast.igbmc.fr/).

Boiero Sanders M., Toret C.P., Guillotin A., Antkowiak A., Vannier T., Robinson R.C, & Michelot A. (2022). Specialization of actin isoforms derived from the loss of key interactions with regulatory factors. The EMBO Journal, 41(5), e107982.

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Tissue RNA Extraction and Amplification

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RNA from a total of five mice from each group was pooled together in three
independent experiments. RNA was extracted from microdissected tissue with a PicoPure RNA
isolation kit following the manufacturer’s suggested protocol. All RNA samples
were linearly amplified with an amplification kit (Invitrogen, Eugene, OR). For LCM, two
rounds of amplification were needed to provide adequate material for analysis using an AMP
kit (Invitrogen, Eugene, OR). The integrity of the RNA was assessed using a bioanalyzer
(model 2100: Agilent technologies, Palo Alto, CA) or using a Nanodrop (Fisher Scientific,
Grand Island, NY).

Kielczewski J.L., Horai R., Jittayasothorn Y., Chan C.C, & Caspi R.R. (2015). Tertiary lymphoid tissue forms in retinas of mice with spontaneous autoimmune uveitis and has consequences on visual function. Journal of immunology (Baltimore, Md. : 1950), 196(3), 1013-1025.

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Hippocampal Gene Expression Profiling in Mice

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RNA was isolated from the whole hippocampus of mice using the RNeasy Mini Kit (Qiagen) according to the manufacturer instructions and quantified using a spectrophotometer (model ND-1000, NanoDrop Technologies). RNA quality was analyzed by determining the RNA Integrity Number (RIN) using a bioanalyzer (model 2100, Agilent Technologies). High-quality RNA (with RIN of at least 6) was used to perform an expression profile of mRNA using the Gene Expression BeadChip Array (Illumina) according to the manufacturer guidelines. In brief, the cRNA library was first generated from mRNA and amplified using an RNA amplification kit (Illumina TotalPrep-96, Ambion). Biotin-linked nucleotides were hybridized to the HumanHT-12-v4-BeadChip (Illumina) for 16 h, sequentially followed by washing, blocking, and staining with Streptavidine-Cy3 following the Expression Direct Hybridization protocol (Illumina). Arrays were scanned using the HiScanSQ System, and images were analyzed by GenomeStudio Gene Expression Module (Illumina). Data were analyzed using one-way ANOVA at p < 0.05. Heatmap visualization of the expression data were performed using the heatmap.2 function from the gplots package (Warnes et al., 2005 ).

Abbah J., Vacher C.M., Goldstein E.Z., Li Z., Kundu S., Talbot B., Bhattacharya S., Hashimoto-Torii K., Wang L., Banerjee P., Scafidi J., Smith N.A., Chew L.J, & Gallo V. (2022). Oxidative Stress-Induced Damage to the Developing Hippocampus Is Mediated by GSK3β. The Journal of Neuroscience, 42(24), 4812-4827.

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Transcriptome Analysis of NF1-C2S, FoxF1, and Control Cells

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Total RNA was prepared from three pools of NF1-C2S-, FoxF1-, or vector control expressing HC11 cells (GenElute Mammalian total RNA kit; Sigma-Aldrich, Stockholm, Sweden). RNA integrity was verified by electrophoresis on a bio-analyzer (model 2100; Agilent, Palo Alto, CA). Five micrograms of each RNA preparation was labeled and hybridized to a Mouse Gene ST 1.0 Array. Hybridization and scanning of the arrays were performed at SCIBLU Microarray Resource Centre (MARC; Lund, Sweden).

Nilsson G, & Kannius-Janson M. (2016). Forkhead Box F1 promotes breast cancer cell migration by upregulating lysyl oxidase and suppressing Smad2/3 signaling. BMC Cancer, 16, 142.

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Whole Exome Sequencing Library Preparation

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In brief, 4 µg of DNA with a concentration of 100 ng/ µL were used for library construction. A liquid chip capture system (Agilent Research Laboratories, Santa Clara, CA, USA) was used to efficiently enrich all human exon regions. High-throughput deep sequencing was subsequently performed on the Illumina (San Diego, CA, USA) platform. An exon kit (SureSelect Human All Exon V6 Kit; Agilent) was used for library construction and capture experiments, and a bioanalyser (Model 2100; Agilent) was subsequently used to verify the library insert size. The Illumina platform was used for sequencing according to the effective concentration of the library and the data output requirements. High-throughput paired-end sequencing (paired-end 150 bp; PE150; Agilent) was performed.

Pap É.M., Farkas K., Tóth L., Fábos B., Széll M., Németh G, & Nagy N. (2020). Identification of putative genetic modifying factors that influence the development of Papillon-Lefévre or Haim-Munk syndrome phenotypes. Clinical and experimental dermatology, 45(5).

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