Srf re luciferase

Manufactured by Promega

The SRF-RE-Luciferase is a reporter gene construct that contains the serum response element (SRF-RE) upstream of the firefly luciferase gene. It is designed for the analysis of serum response factor (SRF) activity in mammalian cells.

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Lab products found in correlation

Sre luciferase, by Promega (2 mentions) Nfat re luciferase, by Promega (2 mentions) Bright glo luciferase assay system, by PerkinElmer (2 mentions) Synergy h1 microplate reader, by Agilent Technologies (1 mentions)

Close spelling variants of this product

SRF-RE

2 protocols using srf re luciferase

CD97-Mediated Transcriptional Regulation Assay

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PDGCs lentivirally infected with a dox-inducible CD97-overexpression or empty vector were transfected with luciferase signaling-reporter plasmids: cAMP response element–Luciferase (Cat# E8471, Promega), SRE-Luciferase (Cat# E1340, Promega), SRF-RE-Luciferase (Cat# E1350, Promega), and NFAT-RE-Luciferase (Cat# E8481, Promega). Twenty-four hours after transfection, cells were reseeded in black 96-well plates at a density of 75,000 cells per well with medium containing doxycycline (or dox-free medium). Forty-eight hours after transfection, cells were lysed and luciferase activity was detected using the Bright-Glo Luciferase assay system (Cat# E2650, CisBio) and a BioTek Synergy H1 microplate reader according to the manufacturer’s protocol.

Ravn-Boess N., Roy N., Hattori T., Bready D., Donaldson H., Lawson C., Lapierre C., Korman A., Rodrick T., Liu E., Frenster J.D., Stephan G., Wilcox J., Corrado A.D., Cai J., Ronnen R., Wang S., Haddock S., Ortiz J.S., Mishkit O., Khodadadi-Jamayran A., Tsirigos A., Fenyö D., Zagzag D., Drube J., Hoffmann C., Perna F., Jones D.R., Possemato R., Koide A., Koide S., Park C.Y, & Placantonakis D.G. (2023). The expression profile and tumorigenic mechanisms of CD97 (ADGRE5) in glioblastoma render it a targetable vulnerability. Cell reports, 42(11), 113374.

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GPR133 Signaling Pathway Analysis

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HEK293T cells were cotransfected with GPR133 overexpression plasmids and either one of the following luciferase signaling-reporter plasmids: cAMP response element–Luciferase (Cat# E8471, Promega), SRE-Luciferase (Cat# E1340, Promega), SRF-RE-Luciferase (Cat# E1350, Promega), NFAT-RE-Luciferase (Cat# E8481, Promega), and NFκB-RE-Luciferase (Cat# E8491, Promega). Twenty-four hours after transfection, cells were reseeded in black 96-well plates at a density of 75,000 cells per well. Forty-eight hours after transfection, cells were lysed and luciferase activity was detected using the Bright-Glo Luciferase assay system (Cat# E2650, CisBio) and a BioTek Synergy H1 microplate reader according to the manufacturer’s protocol.

Frenster J.D., Stephan G., Ravn-Boess N., Bready D., Wilcox J., Kieslich B., Wilde C., Sträter N., Wiggin G.R., Liebscher I., Schöneberg T, & Placantonakis D.G. (2021). Functional impact of intramolecular cleavage and dissociation of adhesion G protein–coupled receptor GPR133 (ADGRD1) on canonical signaling. The Journal of Biological Chemistry, 296, 100798.

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