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Anti flag pab f7425

Manufactured by Merck Group

The Anti-FLAG pAb (F7425) is a polyclonal antibody that recognizes the FLAG epitope tag. The antibody is designed for use in various immunological applications, such as immunoprecipitation, Western blotting, and immunohistochemistry, to detect and purify FLAG-tagged proteins.

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2 protocols using anti flag pab f7425

1

Anti-PRRSV nsp1β Rabbit Antibody Generation

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An anti-PRRSV-nsp1β rabbit pAb specific for NA-PRRSV nsp1β was generated at the Immunological Research Center, University of Illinois at Urbana-Champaign (Urbana, IL). The anti-LV-nsp2/3 antibody was a generous gift from E. Snijder, (Leiden University Medical Center, Leiden, Netherlands). Anti-N protein mAbs (SDOW17) was obtained from E. Nelson (South Dakota State University, Brookings, SD). polyinosinic: polycytidylic [poly (I:C)], DAPI (4′,6-diamidino-2-phenylindol), anti-Flag mAb (F3165), and anti-FLAG pAb (F7425) were purchased from Sigma (St. Louis, MO). Anti-β-actin mAb (sc-47778), anti- HSP90 mAb (sc-69703), and anti-PARP pAb (sc-7150) were purchased from Santa Cruz Biotechnologies Inc. (Santa Cruz, CA). Anti-ISG15 pAb and anti-GFP pAb were purchased from Thermo Scientific (Rockford, IL). Anti-DEPTOR pAb was purchased from Invitrogen (Carlsbad, CA). The peroxidase-conjugated Affinipure goat anti-mouse IgG and peroxidase-conjugated Affinipure goat anti-rabbit IgG were purchased from Jackson Immuno Research (West Grove, PA). Alexa-Flour 488-conjugated and Alexa-Flour 568-conjugated secondary antibodies were purchased from Invitrogen.
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2

Monoclonal Antibodies for Mincle Receptor

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The monoclonal antibody (mAb) to gpMincle (5H4) was established by immunization of Mincle−/− mice [14] (link) with T cell hybridoma cells (2B4) expressing gpMincle. Anti-mMincle mAb (4A9) and anti-hMincle mAb (13D10-H11) were described elsewhere [15] (link), [16] (link). Anti-HA mAbs clone HA124 and TANA2 were from Nacalai tesque and MBL, respectively. Anti-HA polyclonal antibody (pAb) (sc-805) was from SantaCruz. Anti-HA pAb (ab72564) was from abcam. Anti-Flag pAb (F7425) was from Sigma-Aldrich.
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