Vectashield hard set mounting medium with dapi counterstain

Immunofluorescence was performed following standard procedures14 (link). The primary and secondary antibodies used were anti-phospho-histone H3 (p-H3, 1:2000; Ab14955, Abcam), β-catenin (1:250; 610153, BD transduction laboratories), anti-mouse Alexa Fluor 555 IgG (1:500 Invitrogen) and Cy3-streptavidin (1:5000, Jackson ImmunoResearch). Nuclear DNA staining and the mounting were performed with the Vectashield Hard set Mounting Medium with DAPI counterstain (Vector Laboratories). Pictures were taken in an Eclipse 80i microscope and processed with the NIS Elements Advanced Research Software (Nikon).

Immunofluorescence was performed following standard procedures [44 (link)]. The primary and secondary antibodies used were anti-phospho-histone H3 (PH3, 1:2000, Ab14955, Abcam, Cambridge, UK), cleaved-caspase-3 (cC3, 1:500, AF835, R&D Systems, Minneapolis, MN, USA), anti-mouse Alexa Fluor 555 IgG (1:500, Invitrogen, Carlsbad, CA, USA), anti-rabbit Alexa Fluor 488 (1:500, Invitrogen, Carlsbad, CA, USA), and anti-rabbit Alexa Fluor 555 (1:500, Invitrogen, Carlsbad, CA, USA). Nuclear DNA staining and the mounting were performed with the Vectashield Hard set Mounting Medium with DAPI counterstain (Vector Laboratories, Burlingame, CA, USA). Pictures were taken in an Eclipse 80i microscope and processed with the NIS Elements Advanced Research Software (Nikon, Tokyo, Japan).