Chat elisa kit

Curcumin, GW9662, Aβ_1–42, and Griess reagent were purchased from Sigma. Dulbecco's Modified Eagle Medium Nutrient Mixture F-12 (DMEM/F-12), fetal bovine serum (FBS), and Opti- Minimum Essential Medium (MEM) were producted by Gibco. PPARγ siRNA was synthesized by Invitrogen. Lipofectamine LTX and Plus Reagent was produced by Invitrogen. Choline acetyltransferase (ChAT), glial fibrillary acidic protein (GFAP), Iba-1, NF-κB p65, IκBα, and PPARγ antibodies were obtained from Abcam. IL-1β, TNF-α, and COX-2 ELISA kits were purchased from R&D Company. A choline/acetylcholine (Ach) assay kit was supplied by Abcam. A ChAT ELISA kit was obtained from MyBioSource Inc. A PPARγ transcription factor assay kit and PPARγ ligand binding domain (human recombinant) were purchased from Cayman Chemical. A co-immunoprecipitation (Co-IP) kit produced by Pierce was used, and LDH assay kit was supplied by Nanjing Jiancheng Bioengineering Institute.

The test was conducted as described previously^{60 (link)}. The homogenized hippocampus was prepared for measurements of ACh/ChAT levels and AChE activities. The homogenized hippocampus was centrifuged at 12,000×g for 10 min at 4 °C. ACh levels activities were analyzed by the Amplex red ACh assay kit (Invitrogen Life Technologies, A12217, USA) with wavelengths of 560 nm and 590 nm, according to the manufacturer’s protocol. AChE levels activities were analyzed by the AChE Assay kit (BioChain, Z5030044, USA) with wavelengths of 412 nm, according to the manufacturer’s protocol. ChAT levels activities were analyzed by the ChAT Elisa kit (MyBioSource, MBS724080, USA) with wavelengths of 450 nm, according to the manufacturer’s protocol.