Sybr green qrt pcr master mix kit

Manufactured by Tiangen Biotech

The SYBR Green qRT-PCR master mix kit is a reagent used for quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis. The kit contains all the necessary components, including SYBR Green I dye, for the detection and quantification of target RNA sequences.

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Lab products found in correlation

Trizol reagent, by Thermo Fisher Scientific (2 mentions) Primescript rt reagent kit with gdna eraser, by Takara Bio (2 mentions) Nanodrop nd 2000, by Thermo Fisher Scientific (1 mentions) Lightcycler 480 2 real time pcr system, by Roche (1 mentions)

Close spelling variants of this product

SYBR master mix SYBR Green Mix PCR Master Mix QRT-PCR kit SYBR Green kit QRT-PCR SYBR Green

2 protocols using sybr green qrt pcr master mix kit

Quantitative Gene Expression Analysis

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Total RNA extraction was gained by TRIzol reagent (Invitrogen, 15,596,018), purified, eluted in RNase-free water, and subsequently reverse transcribed into cDNA with a PrimeScript RT Reagent Kit with gDNA Eraser (Takara) under the instructions. A SYBR Green qRT-PCR master mix kit (TIANGEN) was utilized to perform qPCR according to the manufacturer’s procedures. All primer sequences are listed in Table 1. Ct values obtained from qPCR were used to calculate the relative expression level of all reported genes via the 2 ^−ΔΔCt method.

Ju D., Liang Y., Hou G., Zheng W., Zhang G., Dun X., Wei D., Yan F., Zhang L., Lai D., Yuan J., Zheng Y., Wang F., Meng P., Wang Y., Yu W, & Yuan J. (2022). FBP1 /miR-24-1/enhancer axis activation blocks renal cell carcinoma progression via Warburg effect. Frontiers in Oncology, 12, 928373.

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Quantitative Analysis of Gene Expression

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Total RNA fractions were isolated using TRIzol reagent (Invitrogen, 15 596 018). With the amount of 1 μg, the total RNA was reversely transcribed into cDNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara). RNA purity and concentration were evaluated by using NanoDrop ND-2000 (Thermo Scientific). qRT-PCR was performed on LightCycler 480 II Real-time PCR system (Roche) using a SYBR Green qRT-PCR master mix kit (TIANGEN) according to the manufacturer's procedure. For qRT-PCR assay, standard vector with 2837 bp was diluted to 50 ng/μl, and its copy number was 1.61 × 10¹⁰ calculated by formula 6.02 × 10²³ × concentration (50 ng/μl) × 10^–9 / (2837 × 660). Next, the standard was diluted into four different gradient concentrations to abtain the standard curve. All primer sequences were listed in Supplementary Table S1. The 2^−ΔΔCt method was applied to detect the relative expression of each gene.

Liang Y., Lu Q., Li W., Zhang D., Zhang F., Zou Q., Chen L., Tong Y., Liu M., Wang S., Li W., Ren X., Xu P., Yang Z., Dong S., Zhang B., Huang Y., Li D., Wang H, & Yu W. (2021). Reactivation of tumour suppressor in breast cancer by enhancer switching through NamiRNA network. Nucleic Acids Research, 49(15), 8556-8572.

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