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Firepol qpcr master mix

Manufactured by Solis BioDyne
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Firepol qPCR Master Mix is a ready-to-use solution for quantitative real-time PCR (qPCR) applications. It contains all the necessary components, including a DNA polymerase, buffer, and fluorescent dye, required for qPCR reactions.

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Lab products found in correlation

Goscript reverse transcriptase kit, by Promega (1 mentions) 7900ht fast real time pcr system, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Lightcycler 480 system, by Roche (1 mentions)

Spelling variants of this product

FIREPol Master Mix (45 citations) HOT FIREPol EvaGreen qPCR master mix (2 citations)

2 protocols using firepol qpcr master mix

1

Liver RNA Extraction and RT-qPCR Analysis

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RNA was isolated from shock frozen liver samples using the RNeasy Mini kit (Qiagen) according to the manufacturer’s instructions. RNA was reverse transcribed to cDNA using random primers and GoScript Reverse Transcriptase kit (Promega). RT-qPCR was performed using Firepol qPCR Master Mix (Solis BioDyne) and analyzed by 7900HT Fast Real-Time PCR System (Applied Biosystems). Fold changes were calculated using the delta Ct method. Used primers are listed in Table S4.
Böck D., Rothgangl T., Villiger L., Schmidheini L., Matsushita M., Mathis N., Ioannidi E., Rimann N., Grisch-Chan H.M., Kreutzer S., Kontarakis Z., Kopf M., Thöny B, & Schwank G. (2022). In vivo prime editing of a metabolic liver disease in mice. Science translational medicine, 14(636), eabl9238.
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2

Quantitative PCR Analysis of cDNA

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Reverse transcribed cDNA was quantified by qPCR after the enzymatic reactions were stopped. qPCR was performed using the FIREPol qPCR Master Mix (Solis BioDyne) and analyzed using a Lightcycler 480 system (Roche). Quantifications of virtual products were performed with the formula: 10((Ct-14.87/-3.48) *1'000'000.
Weber Y., Böck D., Ivașcu A., Mathis N., Rothgangl T., Ioannidi E.I., Blaudt A.C., Tidecks L., Vadovics M., Muramatsu H., Reichmuth A., Marquart K.F., Kissling L., Pardi N., Jinek M, & Schwank G. (2024). Enhancing prime editor activity by directed protein evolution in yeast. Nature Communications, 15, 2092.
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