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Cd1d pe

Manufactured by BD

CD1d-PE is a fluorescently-labeled monoclonal antibody that binds to the CD1d protein. CD1d is a major histocompatibility complex (MHC) class I-like molecule that presents lipid antigens to Natural Killer T (NKT) cells. The PE (Phycoerythrin) fluorescent label allows for the detection and quantification of CD1d-expressing cells using flow cytometry.

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2 protocols using cd1d pe

1

Cell Surface Antigen Expression Analysis

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Monoclonal antibodies specific for CD95 (Fas)-PE, CD54 (ICAM-1)-PE, CD1d-PE, and HLA-ABC-PE were obtained from BD: Becton, Dickinson and Company (San Jose, CA). The monoclonal antibody specific for CD273 (PL-L2)-PE was obtained from BioLegend (San Diego, CA). The cultured cells were stained for expression of cell-surface antigens and flow cytometry was performed using a FACSCaliber instrument (BD) and analyzed with CellQuest software (BD).
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2

Comprehensive Immune Cell Profiling

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Bone marrow, peripheral blood, and splenic B cells from cCD79 and wild type mice were analyzed using the following antibodies: hCD79A-PE (R&D Systems, FAB69201P); hCD79B-PE (abcam, ab33295); mCD79B-AF488 ([HM79], made in house); hCD79A-AF647 ([Curly-14], made in house); B220-BV786 (BD, 563894); B220-APC (BD, 103212); B220-PE (BD, 561878); CD43 ACP-Cy7 (BD, 562866); BP1-PE (BD, 553735); CD24-APC (BioLegend, 101814); IgD-FITC (BioLegend, 405704); IgM-BUV396 (BD, 564025); CD19-FITC (BioLegend, 152404); CD93-APC (BioLegend, 136510); CD23-FITC (BD, 561772); CD1d-PE (BD, 553846); CD95-PE (BD, 554258); GL7-FITC (BD, 553666); fab anti-mIgG (H+L)-AF647 (Jackson ImmunoResearch, 115–606-072); phospho-Syk (Y352)-PE (BD, 557881); PTEN-PE (BD, 560002); mouse IgG1 isotype control-PE (BD, 559320).
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