Human primary neurogliocyte icell-n009 and four glioma cell lines (U87, U251, LN229, HS683) were purchased from Shanghai Cell Bank (Shanghai, China). For CIP2A depletion, the cells were transfected by lentivirus-CIP2A-shRNA or lentivirus-CIP2A-NC (GenePharma, China). Transfection efficiency on CIP2A knockdown was tested by qRT-PCR.
Hs683
Manufactured by Shanghai Cell Bank
Sourced in China
The HS683 is a cell culture incubator designed for maintaining optimal growth conditions for cell lines. It features precise temperature, humidity, and CO2 control to provide a stable and consistent environment for cell culture applications.
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Lab products found in correlation
2 protocols using hs683
1
Lentiviral CIP2A Knockdown in Glioma Cells
2
Immunohistochemistry and RT-qPCR Analysis of Glioma Samples
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15 glioma tissues and 11 normal brain samples were collected from Xiangya Hospital, Central South University between January 2016 and January 2022. Gliomas were classified according to 2016 WHO classification: five WHO II cases, four WHO III cases, and six WHO IV cases. The glioma tissues of different WHO grades and normal brain tissues were analyzed by immunohistochemistry staining (IHC) and 11 pairs of glioma samples and normal brain samples were analyzed by RT-qPCR. This study was approved by the Ethics Committee of Xiangya Hospital of Center South University. Human glioma cells HA 1,800, A172, U87, U251, HS683, and LN229 were purchased from Shanghai Cell bank. All cells were cultured in a humidified atmosphere containing 5% CO2/95% air at 37°C. Dulbecco’s Modified Eagle’s Medium (high glucose) with 10% fetal bovine serum (Bovogen) and 1% penicillin/streptomycin was applied to culture cells.
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