HPLC analysis was performed on a Shimadzu 20A HPLC, using an Aminex HPX-87H column with a Micro-Guard cation H+ guard column (Bio-Rad, Hercules, CA) at 55°C. Samples were analyzed using a flow rate of 0.6 ml/min, in 5 mM sulfuric acid with a 30-min run time. Eluent was prepared by diluting a 50% HPLC-grade sulfuric acid solution (Fluka) in Milli-Q water and then degassing the solution at 37°C for 3 to 5 days before use. Compounds of interest were detected by refractive index (RID-20A). Samples were prepared by centrifuging 1-ml samples taken from flask growth for 10 min at 13,000 × g in a microcentrifuge (Minispin Plus; Eppendorf) to remove cells. The supernatant was removed and transferred to a 2.0-ml glass HPLC vial. Standards were prepared at concentrations of 1, 2, 5, 10, and 20 mM for d ,l -lactate, NAG, and sodium acetate. Samples were maintained at 10°C by an autosampler throughout analysis.
20a hplc
Manufactured by Bio-Rad
The 20A HPLC is a high-performance liquid chromatography system designed for analytical applications. It features a binary solvent delivery system, a UV-Vis detector, and a temperature-controlled column compartment. The 20A HPLC provides precise and reliable separation, identification, and quantification of a wide range of chemical compounds.
Automatically generated - may contain errors
2 protocols using 20a hplc
1
HPLC Analysis of Metabolite Concentrations
2
HPLC Analysis of 2,3-Butanediol and Acetoin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HPLC analysis was performed on a Shimadzu 20A HPLC, using an Aminex HPX-87H (BioRad, Hercules, CA) column with a Micro-guard Cation H + guard column (BioRad, Hercules, CA) at 65°C. Compounds of interest were separated using a 0.6 mL/min flow rate, in 5 mM sulfuric acid with a 30-minute run time. Eluent was prepared by diluting a 50% HPLC-grade sulfuric acid solution (Fluka) in Milli-Q water and degassing the solution at 37°C for 3-5 days before use.
Compounds of interest were detected by a refractive index detector (Shimadzu, RID-20A) maintained at 60°C. Samples were prepared by centrifuging 1-mL samples taken from the working electrode chambers for 10 minutes at 13,000 rpm in a microcentrifuge (Minispin Plus, Eppendorf) to remove cells. The supernatant was removed and transferred to a 2.0-mL glass HPLC vial (Vial: Restek, 21140; Cap: JG Finneran, 5395F09). Mixed standards of 2,3butanediol and acetoin were prepared at concentrations of 1, 2, 5, 10, and 15 mM. Samples were maintained at 10°C by an auto-sampler (Shimadzu, SIL-20AHT) throughout analysis. Acetoin and 2,3-butanediol concentrations in the samples were determined using linear calibration curves based on the external standards.
Compounds of interest were detected by a refractive index detector (Shimadzu, RID-20A) maintained at 60°C. Samples were prepared by centrifuging 1-mL samples taken from the working electrode chambers for 10 minutes at 13,000 rpm in a microcentrifuge (Minispin Plus, Eppendorf) to remove cells. The supernatant was removed and transferred to a 2.0-mL glass HPLC vial (Vial: Restek, 21140; Cap: JG Finneran, 5395F09). Mixed standards of 2,3butanediol and acetoin were prepared at concentrations of 1, 2, 5, 10, and 15 mM. Samples were maintained at 10°C by an auto-sampler (Shimadzu, SIL-20AHT) throughout analysis. Acetoin and 2,3-butanediol concentrations in the samples were determined using linear calibration curves based on the external standards.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!