Anti pd l1 pe clone 10f 9g2

Spinning disk confocal microscopy was performed on all animals from the liver metastasis model and splenectomized control mice using the customized Olympus IX81-inverted microscope for visualizing CT26-iRFP tumors and CXCR6-gfp þ cells in the liver. The microscope was equipped with an Olympus focus drive that had a motorized xyz stage (Applied Scientific Instrumentation, MS-2000 with piezo-z insert) and a motorized objective turret holding three objective lenses: 4Â/0.16 UPLANSAPO, 10Â/0.40 UPLANSAPO, and 20Â/0.70 UPLANSAPO. The objective lenses were connected to a light path (WaveFx; Quorum Technologies) that was linked to a Yokogawa CSU-10 head (Yokogawa Electric Corporation). The acquired images were recorded with an EM-CCD camera. The image acquisition software used was Volocity (Quorum Technologies). A detailed description of the procedure and preparation of the animals has been published in ref. 38 . For in vivo imaging of PD-1, PD-L1, and Kupffer cells in Cxcr6 gfp/þ knock-in mice, the following antibodies were used: anti-PD-1-APC (clone 29F.1A12, Biolegend), anti-PD-1-isotype control-APC (clone RTK2758, Biolegend), anti-PD-L1-PE (clone 10F.9G2, Biolegend), anti-PD-L1-isotype control-APC (clone RTK4530, Biolegend), and anti-F4/ 80-AF750 (clone BM8, AbLab).