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4 protocols using tween 80

1

Pharmacological Characterization of PEA

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Palmitoylethanolamide, MK886, a PPARα antagonist, and GW6471, a PPARα antagonist, were purchased from Cayman Chemical (Ann Arbor, MI, United States). PEA was dissolved in dimethyl sulfoxide (Wako Pure Chemical Industries, Osaka, Japan) for the in vitro experiments, and dissolved in a vehicle composed of Tween 80 (Kanto Chemical, Tokyo, Japan), dimethyl sulfoxide, and phosphate-buffered saline (PBS, Life Technologies, Carlsbad, CA, United States) (1:0.5:18.5 by volume) for the in vivo experiments.
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2

Lutein-Cyclodextrin Formulation Development

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The lutein (Lutemax) is kindly provided by DKSH (Taipei, Taiwan). Cyclodextrins are purchased from Wako Pure Chemical Industries (Osaka, Japan). Transcutol HP is obtained from Gattefossé (Lyon, France). Tween 80 and Span 60 are supplied by Kanto Chemical (Tokyo, Japan). All other chemicals and reagents are purchased from Sigma-Aldrich unless otherwise stated.
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3

OEA and PPAR-α Antagonist Study

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OEA and MK866, a PPAR-α antagonist, were purchased from Cayman Chemical (Ann Arbor, MI, USA). OEA was dissolved in dimethyl sulfoxide (DMSO, Wako Pure Chemical Industries, Osaka, Japan) for the in vitro experiments and was dissolved in a vehicle composed of Tween 80 (Kanto Chemical, Tokyo, Japan), DMSO, and phosphate-buffered saline (PBS, Life Technologies, Carlsbad, CA, USA) (1:0.5:18.5 by volume) for the in vivo experiments.
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4

Evaluating Ivermectin's Anticoagulant Effects

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Ethanol, polyethylene glycol (PEG) 400, and hydrochloric acid were purchased from Fujifilm Wako Pure Chemical (Osaka, Japan). Tween 80 was purchased from Kanto Chemical (Tokyo, Japan). Metallo Assay Zinc LS was purchased from Metallogenics (Chiba, Japan). Hamster D-Dimer (D2D) ELISA kits were purchased from My BioSource (San Diego, CA). Ivermectin was purchased from Sigma Aldrich (St. Louis, MO).
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