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Protoscript m mulv

Manufactured by New England Biolabs
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ProtoScript M-MuLV is a reverse transcriptase enzyme derived from Moloney Murine Leukemia Virus. It is used to catalyze the conversion of RNA to complementary DNA (cDNA) in reverse transcription reactions.

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Lab products found in correlation

Abi steponeplus real time pcr system, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Sybr premix ex taqtm, by Takara Bio (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

ProtoScript M-MuLV First Strand cDNA Synthesis Kit (47 citations) M-MuLV (19 citations) ProtoScript M-MuLV Taq RT-PCR Kit (19 citations) ProtoScript M-MuLV First-Strand Synthesis Kit (3 citations) Protoscript® M-MuLV II First Strand cDNA Synthesis Kit (2 citations) ProtoScript Moloney murine leukemia virus (M-MuLV) first-strand cDNA synthesis kit (2 citations)

2 protocols using protoscript m mulv

1

Gene Expression Analysis Protocol

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Total RNA was isolated from cells or tissues using TRIzol reagent (Invitrogen, Shanghai, China). cDNA for was synthesized using ProtoScript M-MuLV (NEB,USA). The DNA standards were performed using pspt18 vector (Promega, USA) as previously described40 (link), and primer sequences were listed in Supplementary Table 1. Real-time PCR was performed using SYBR Premix Ex TaqTM (TaKaRa, Dalian, China) on ABI StepOne Plus™ Real-Time PCR System (Applied Biosystems, USA). Relative expression was analyzed by using 2−ΔΔCt and are referred to the control treatment using RPLP0 as a reference gene. The primer sequences were listed in Supplementary Table 2.
Chu W., Wei W., Han H., Gao Y., Liu K., Tian Y., Jiang Z., Zhang L, & Chen J. (2017). Muscle-specific downregulation of GR levels inhibits adipogenesis in porcine intramuscular adipocyte tissue. Scientific Reports, 7, 510.
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2

Arterial RNA Extraction and qPCR

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Total RNA was extracted from either whole arteries or ~ 200-300 isolated selected arterial myocytes using TRIzol (Life Technologies) or the Absolutely RNA Nanoprep kit (Stratagene), respectively. First-strand cDNA was generated from 1-5 ng of total RNA using Protoscript M-MULV (New England Biolabs). PCR was performed on first-strand cDNA using primers sequences shown in Online Table I. PCR products were separated on 1.5% agarose gels.
Evanson K.W., Bannister J.P., Leo M.D, & Jaggar J.H. (2014). LRRC26 is a Functional BK Channel Auxiliary γ Subunit in Arterial Smooth Muscle Cells. Circulation research, 115(4), 423-431.
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