Discovery glycan solid phase extraction tubes

All chemicals and reagents were at least
analytical reagent grade and used without further purification. Rapid
PNGase F and Rapid PNGase F Buffer were supplied by New England Biolabs
(Ipswich, USA). Human alpha-1-acid glycoprotein (hAGP, ≥95%),
Discovery Glycan solid phase extraction (SPE) tubes, TFA (≥99%),
procainamide hydrochlorid (≥98%), and both trisaccharide standards
6′/3′-Sialyl-N-acetyllactosamine were
purchased from Sigma-Aldrich (St. Louis, USA). The fucosylated standard
3′-Sialyl-Lewis X was supplied by Biosynth Carbosynth (UK).
Hypercarb SPE tubes were purchased from Thermo Fisher Scientific (Waltham,
USA). Ammonium formate (>99%) was obtained from VWR International
(Radnor, USA). All solvents (acetonitrile, methanol, and water) were
LC-MS grade and purchased from Sigma-Aldrich (St. Louis, USA).

All chemicals and reagents were at least analytical reagent grade and used without further purification. Rapid™ PNGase F and Rapid™ PNGase F Buffer were supplied by New England Biolabs (Ipswich, USA). Immunoglobulin from human serum (hIgG, ≥ 95%), human alpha-1-acid glycoprotein (hAGP, ≥ 95%), dextran Mw5000, dextran Mw1000, Discovery Glycan solid-phase extraction (SPE) tubes, and procainamide hydrochloride (≥ 98%) were purchased from Sigma-Aldrich (St. Louis, USA). Ammonium formate (> 99%) was obtained from VWR International (Radnor, USA). All solvents (acetonitrile, water) were LC–MS grade and purchased from Sigma-Aldrich (St. Louis, USA).