Caspase 3 3g2 mouse mab

Flash column chromatographic separations were accomplished on Biotage Isolera Four system (Biotage, Uppsala, Sweden). NMR was recorded on DRX-400 and DRX-500 NMR spectrometers (Bruker, Rheinstetten, Germany). ESI-, APCI- and HR ESI-MS were recorded on Agilent 1290 LC-MS or Agilent G6530 TOF MS spectrometer (Agilent Technologies Inc., California, USA). Western blot results were visualized on Mini-HD9-Auto Biomolecular imager (Leader Oriental Technology. LTD, Beijing, China). Dichlone and flavonoids were purchased from Fisher Scientific (Fair Lawn, NJ, USA) and Ark Pharm, Inc. (Arlington Heights, IL, USA). Cell lines were gotten from the American Type Culture Collection (Manassas, VA). Caspase-3 (3G2) mouse mAb, caspase-3 antibody, cleaved caspase-3 (Asp175) (5A1E) rabbit mAb, PARP (46D11) rabbit mAb and horseradish peroxidase (HRP) were purchased from Cell Signaling Technology, Inc (Danvers, MA, USA).

SBC-3 cells (cell concentration: 5
× 10⁵ cells/mL) were cultured in a six-well flat-bottom
plate and treated with EtOH/H₂O (1:1), 10 μM cisplatin,
or 15 μM 1 for 24 h. The Western blotting analysis
was carried out by the same procedures as previously reported.^{26 (link)} The following antibodies were recruited: β-actin
(8H10D10 Mouse mAb, product number 3700, 1:1000; Cell Signaling Technology,
Danvers, MA, USA), caspase-8 (1C12 Mouse mAb, product number 9746,
1:1000; Cell Signaling Technology), caspase-9 (C9 Mouse mAb, product
number 9508, 1:2000; Cell Signaling Technology), caspase-3 (3G2 Mouse
mAb, product number 9668, 1:1000, Cell Signaling Technology), PARP
(46D11 Rabbit mAb, product number 9532, 1:1000; Cell Signaling Technology),
Bax (2D2 Mouse mAb, product number 89477, 1:1000; Cell Signaling Technology),
Bcl-2 (124 Mouse mAb, product number 15071, 1:1000; Cell Signaling
Technology), anti-rabbit IgG, horseradish peroxidase (HRP)-linked
antibody (product number 7074, 1:10000; Cell Signaling Technology),
and anti-mouse IgG, HRP-linked antibody (product number 7076, 1:10000;
Cell Signaling Technology). The signals were detected using ECL Prime
Western Blotting Detection Reagents (GE Healthcare, Boston, MA, USA),
and photographed by a LAS-3000 luminescent image analyzer (FUJIFILM,
Tokyo, Japan).