Caspase 3 3g2 mouse mab
Caspase-3 (3G2) mouse mAb is a monoclonal antibody that recognizes the cleaved form of caspase-3, a key effector of apoptosis. It is used for the detection of activated caspase-3 in various applications.
Lab products found in correlation
3 protocols using caspase 3 3g2 mouse mab
Chromatographic Separation and MS Analysis
Apoptosis Signaling Pathway Analysis
× 105 cells/mL) were cultured in a six-well flat-bottom
plate and treated with EtOH/H2O (1:1), 10 μM cisplatin,
or 15 μM
was carried out by the same procedures as previously reported.26 (link) The following antibodies were recruited: β-actin
(8H10D10 Mouse mAb, product number 3700, 1:1000; Cell Signaling Technology,
Danvers, MA, USA), caspase-8 (1C12 Mouse mAb, product number 9746,
1:1000; Cell Signaling Technology), caspase-9 (C9 Mouse mAb, product
number 9508, 1:2000; Cell Signaling Technology), caspase-3 (3G2 Mouse
mAb, product number 9668, 1:1000, Cell Signaling Technology), PARP
(46D11 Rabbit mAb, product number 9532, 1:1000; Cell Signaling Technology),
Bax (2D2 Mouse mAb, product number 89477, 1:1000; Cell Signaling Technology),
Bcl-2 (124 Mouse mAb, product number 15071, 1:1000; Cell Signaling
Technology), anti-rabbit IgG, horseradish peroxidase (HRP)-linked
antibody (product number 7074, 1:10000; Cell Signaling Technology),
and anti-mouse IgG, HRP-linked antibody (product number 7076, 1:10000;
Cell Signaling Technology). The signals were detected using ECL Prime
Western Blotting Detection Reagents (GE Healthcare, Boston, MA, USA),
and photographed by a LAS-3000 luminescent image analyzer (FUJIFILM,
Tokyo, Japan).
Immunodetection of Apoptotic Markers
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!