Genomic DNA was extracted from paraffin-embedded tissue specimens of surgically resected gastric cancers. Tumors areas were marked on hematoxylin and eosin stained slides by one pathologist (Y. Baba). Genomic DNA was extracted from tumor lesions enriched with neoplastic cells, without adjacent normal tissue, using FFPE Kits (Qiagen, Duesseldorf, Germany).
Ffpe kits
Manufactured by Qiagen
Sourced in Germany
FFPE Kits are a set of products designed to extract and purify nucleic acids (DNA, RNA) from formalin-fixed, paraffin-embedded (FFPE) tissue samples. These kits provide a standardized and efficient method for isolating high-quality nucleic acids from FFPE samples, which are commonly used in pathology and research applications.
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Lab products found in correlation
2 protocols using ffpe kits
1
Extracting Gastric Cancer DNA from FFPE
2
Metastatic Tumor DNA and RNA Isolation
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DNA and RNA isolation from the freshly frozen cryostat-microdissected metastases (n = 9; Fig. 3B) was performed with phenol–chloroform extraction or QIAGEN column extraction. A noncancerous kidney sample was used as germline reference control. Cancer cell fraction (CCF) estimated by visual analysis of hematoxylin and eosin–stained dissected tumor sections and from examination of allelic fractions was between 75% and 90% (mean CCF was 84.6%) for all tumor samples. WGS was performed for all nine metastatic samples and RNA-seq was performed in six of the same metastatic tissue materials (alternating sections used for DNA or RNA isolation). Laser microdissection of selected foci (Fig. 1 B) from the prostate removed 5 yr before death was performed using Arcturus and MMI dissection systems. DNA isolation was performed using QIAGEN FFPE kits.
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