Dako edta target retrieval solution

Human BM core biopsy slides (IRB #57532) were obtained from the Duke University Department of Pathology. SDF-1 and Ki67 immunohistochemistry staining was performed under the following conditions: SDF-1 antigen retrieval was performed with Dako EDTA Target Retrieval solution (Dako North America Inc.). Slides were stained with anti–SDF-1 (clone 79018, R&D Systems) at a concentration of 15 μg/ml. Dako Envision System-HRP (DAB), for use with mouse (#K4006, Dako), was used as a secondary antibody. For Ki67, antigen retrieval was performed with Dako EDTA Target Retrieval solution (Dako North America Inc.) Slides were stained with anti-Ki67 (clone ab15580, Abcam) at a concentration of 10 μg/ml. Dako Envision System-HRP (DAB), for use with rabbit (#K4011, Dako), was used as a secondary antibody. Whole slide images of human BM core slides were obtained with an Axio Observer Z1 microscope, Axiocam ICc1 color camera, and Axiovision 4.8 software. The distances of individual human BCCs, megakaryocytes, and adipocytes from sinusoidal vasculature and trabecular bone were measured by a clinical hematopathologist (C.M.M.) using the ImageJ software (version 1.49, NIH) and whole-slide images.