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Silica gel 60 f

Manufactured by Merck Group
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Sourced in Germany

Silica gel 60 F is a type of adsorbent material used in various laboratory applications. It is composed of small, porous particles of silica that have a high surface area, making them effective at adsorbing and trapping a variety of substances. This product is commonly used in column chromatography, thin-layer chromatography, and other separation techniques to purify or isolate specific compounds from complex mixtures.

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Lab products found in correlation

Streptomycin sulphate, by Merck Group (1 mentions) Anhydrous sodium sulphate, by Merck Group (1 mentions) Chloroacetyl chloride, by Merck Group (1 mentions) Nystatin, by Merck Group (1 mentions) Pet ether, by Merck Group (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Chloroform, by Merck Group (1 mentions) Ethyl acetate, by Merck Group (1 mentions) 1 octanol, by Merck Group (1 mentions) Potassium carbonate, by Merck Group (1 mentions) Silica gel 60 f254, by Merck Group (1 mentions) Tlc plate, by Merck Group (1 mentions) Silica gel 60, by Merck Group (1 mentions)

Close spelling variants of this product

Precoated silica gel 60 F 254 aluminum sheets 0.2 mm precoated silica gel aluminium sheets (60 F-254). HPTLC plates silica gel 60 F 254 0.2 mm silica gel 60 F-254 Al-plates Silica gel 60 F-254 TLC sheets Silica Gel 60 F-254 thinlayer plates Precoated silica gel 60 F-254 glass plates TLC silica gel 60 F 254 aluminum plates Silica gel 60 Silica gel

5 protocols using silica gel 60 f

1

Synthesis and Characterization of Novel Organosulfur Compounds

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Chemicals and reagents consumed in the syntheses were obtained
commercially and utilized without further purification unless notified.
Sodium methoxide in methanol, N′,N′-dimethyl formamide (anhydrous), N-acetyl-l-cysteine, ethyl acetate, pet ether, chloroacetyl
chloride, 1-octanol, sodium sulphate anhydrous, potassium carbonate,
TFA, acetonitrile, methanol, DCM, chloroform, and water were purchased
from E. Merck Ltd. (India). Among these, solvents were purchased of
HPLC grade. Dulbecco’s modified Eagle’s medium (DMEM),
penicillin, streptomycin sulphate, nystatin, and trypsin–ethylenediaminetetraacetic
acid (EDTA) (10×) were bought from Sigma, USA. Foetal bovine
serum was obtained from GIBCO.
The reactions demanding anhydrous
conditions were carried under
an inert N2 atmosphere. Anhydrous methanol was prepared
by using 4 Å molecular sieves. The reaction temperatures mentioned
denote the temperature of the reaction vessel’s water bath
and not the reaction vessel itself. Aluminium-coated silica gel sheets
(silica gel 60 F, Merck, Germany) were used to run TLC and seen by
potassium permanganate developing solution. ITLC was done to assess
radiocomplexation yield and percentage of radiochemical purity.
Prakash S., Hazari P.P., Meena V.K, & Mishra A.K. (2018). Radiolabeling and Preclinical Evaluation of a New S-Alkylated Cysteine Derivative Conjugated to C-Substituted Macrocycle for Positron Emission Tomography. ACS Omega, 3(6), 6497-6505.
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2

Analytical Characterization of Organic Compounds

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All reagents were used directly as obtained commercially unless otherwise noted. All moisture-sensitive reactions were performed under an argon atmosphere using oven-dried glassware and anhydrous solvents. Column flash chromatography was carried out using E. Merck silica gel 60F (230–400 mesh). Analytical thin-layer chromatography (TLC) was performed on aluminum sheets coated with silica gel 60 F254 (0.25 mm thickness, E. Merck, Darmstadt, Germany).
1H NMR spectra were recorded with a Bruker-400 NMR spectrometer at nominal resonance frequencies of 400 MHz, in CDCl3 or DMSO-d6 (referenced to internal Me4Si at δH 0 ppm). The chemical shifts (δ) were expressed in parts per million (ppm). First order J values were given in Hertz. Splitting patterns are described as singlet (s), doublet (d), triplet (t), quartet (q), and broad (br).
The radio-high performance liquid chromatography (HPLC) system consisted of two Varian ProStar pumps, a single Rheodyne Model 7725i manual injector, a ProStar 325 UV-Vis variable wavelength detector, and an in-line BioScan Flow-Count radioactivity detector. All HPLC chromatograms were recorded with Varian Galaxy software (version 1.8). Analytical and semi-preparative chromatography was performed using Phenomenex Luna C-18 10 μm columns (analytical 4.6 × 250 mm and semi-preparative 10 × 250 mm).
Gao Y., Mease R.C., Olson T.T., Kellar K.J., Dannals R.F., Pomper M.G, & Horti A.G. (2014). [125I]Iodo-ASEM, a specific in vivo radioligand for α7-nAChR. Nuclear medicine and biology, 42(5), 488-493.
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3

Thin-Layer Chromatography Purification Procedure

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Thin-layer chromatography
(TLC) was
performed on Merck TLC plates (aluminum-based) silica gel 60 F 254.
Purification was carried out using column chromatography on silica
gel 60 (Merck). Petroleum ether as the eluent had the boiling range
of 60–70 °C.
Söftje M., Weingartz T., Plarre R., Gjikaj M., Namyslo J.C, & Kaufmann D.E. (2021). Surface Tuning of Wood via Covalent Modification of Its Lignocellulosic Biopolymers with Substituted Benzoates—A Study on Reactivity, Efficiency, and Durability. ACS Omega, 6(49), 33542-33553.
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4

Flavonoid Extraction and TLC Analysis

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Extraction of Flavonoids : Powdered sample (5g) was defatted first after that extracted with methanol in soxhlet apparatus for two hours and solvent was evaporated the residue was collected and stored in well closed container.
For TLC dried residue was dissolved in methanol and the standard flavonoid (quercetin 98%w/w) also dissolved in methanol (2mg/ml). Both the samples were loaded on silica gel 60 F (E Merck). The plate was developed using ,ethyl acetate :glacial acetic acid :formic acid :water (5 : 0.5: 0.5 :1.3). The plate was sprayed with NP/PEG.
Tahir S.N.M. (2021). Pharmacognostical Studies on Leaves of Madhuca longifolia (koen) Macbr. International Journal of Pharmaceutical Sciences Review and Research, 71(1).
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5

Thin-Layer Chromatography Analysis

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TLC analysis was performed on Merck silica gel 60 F 254 precoated aluminium sheets with fluorescence indicator F 254 . In addition, detection of the intrinsic tetrapyrrole fluorescence was performed with UV light at 366 nm.
Staegemann M.H., Gräfe S., Haag R, & Wiehe A. (2016). A toolset of functionalized porphyrins with different linker strategies for application in bioconjugation. Organic & biomolecular chemistry, 14(38).
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