Primary murine hepatocytes were incubated with rabbit-polyclonal-anti SLC22A1 (GeneTex International Corporation, Irvine, CA, USA) as primary antibody after preincubation with hydrogen peroxide for blocking of endogenous peroxidase. Endogenous biotin was blocked with the Avidin-Biotin Blocking kit (Vector Laboratories, Burlingame, CA, USA) and contaminating proteins were inhibited by ROTI®-Immunoblock solution (ROTH, Karlsuhe, Germany). After incubation with the secondary antibody (goat anti-rabbit IgG-Biotin, 1:1000; DAKO Cytomation, Hamburg, Germany) the TSA™ Cyanine system (Perkin Elmer, Waltham, MA, USA) was added. For negative control the primary antibody was omitted. The images were evaluated under a fluorescence microscope (Olympus BX51, Olympus U-RFL-T).
Roti immunoblock solution
Manufactured by Carl Roth
Sourced in Germany
ROTI®-Immunoblock solution is a ready-to-use blocking buffer designed for immunochemical applications. It is formulated to reduce non-specific binding in western blotting, ELISA, and other immunoassays.
Automatically generated - may contain errors
2 protocols using roti immunoblock solution
1
Immunohistochemistry of SLC22A1 in Murine Hepatocytes
2
Immunohistochemical Analysis of Hnf4α in Murine Hepatocytes
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Primary murine hepatocytes were incubated with rabbit-polyclonal-anti Hnf4α (Bioss Antibodies Inc.) as the primary antibody after preincubation with hydrogen peroxide for blocking of endogenous peroxidase. Endogenous biotin was blocked with the Avidin-Biotin Blocking kit (Vector Laboratories) and contaminating proteins were inhibited by ROTI®-Immunoblock solution (ROTH). After incubation with the secondary antibody (goat anti-rabbit IgG-Biotin, 1:1,000; Dako Cytomation), the TSA™ Cyanine system (Perkin Elmer) was added. For the negative control, the primary antibody was omitted. The images were evaluated under a fluorescence microscope (Olympus BX51, Olympus U-RFL-T).
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