Mouse anti syk

Manufactured by Santa Cruz Biotechnology

Mouse anti-Syk is a primary antibody that recognizes the Syk (Spleen Tyrosine Kinase) protein. Syk is a cytoplasmic tyrosine kinase that plays a crucial role in signal transduction pathways involved in various cellular processes, such as immune response, cell differentiation, and proliferation. This antibody can be used for the detection and analysis of Syk expression in different cell types and tissues.

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Lab products found in correlation

Coomassie plus protein reagent, by Thermo Fisher Scientific (1 mentions) Rabbit anti src pan, by Thermo Fisher Scientific (1 mentions) Protein assay, by Bio-Rad (1 mentions) Mouse anti β actin, by Abmart (1 mentions) Mouse anti β tubulin, by Abmart (1 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)

2 protocols using mouse anti syk

Western Blot Analysis of Phosphorylated Signaling Proteins

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For validation by Western blotting, four samples corresponding to four biological replicates from different cohorts of healthy donors were analysed per condition and experimental setting.
Proteins were precipitated from lysates in 20% trichloroacetic acid in acetone as previously described 15 , and protein pellets were resuspended in 50μl of sample buffer (65mM CHAPS, 5M urea, 2M thiourea, 0.15M NDSB-256, 30mM Tris, 1mM sodium vanadate, 0.1mM sodium fluoride, and 1mM benzamidine). Protein quantitation was done with Coomassie Plus protein reagent (Thermo Scientific) following manufacturer's protocol.
Protein separation by SDS-PAGE and subsequent immunoblotting were carried out as indicated in the Supplemental Methods. The following primary antibodies were used: rabbit anti-human p-PLCγ2 (Y759) (MAB7377, R&D systems) dilution 1/300; rabbit anti-p-Syk (Y525+Y526) (ab58575, Abcam) dilution 1/1000; rabbit anti-p-Src(Y418) (44660G, Fisher Scientific), dilution 1/1000; mouse anti-PLCγ2 (sc-5283, Santa Cruz Biotechnology) dilution 1/500; mouse anti-Syk (sc-1240, Santa Cruz Biotechnology) dilution 1/1000; rabbit anti-Src pan (44-656G,Invitrogen), dilution 1/1000. Further information on the statistical analysis can be found in the Supplemental Methods.

Izquierdo I., Barrachina M.N., Hermida-Nogueira L., Casas V., Morán L.A., Lacerenza S., Pinto-Llorente R., Eble J.A., de Los Ríos V., Domínguez E., Loza M.I., Casal J.I., Carrascal M., Abián J, & García A. (2020). A Comprehensive Tyrosine Phosphoproteomic Analysis Reveals Novel Components of the Platelet CLEC-2 Signaling Cascade. Thrombosis and haemostasis, 120(2).

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Western Blot Analysis of Glucose Transporters

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Protein solutions from three mice in each group were denatured with 5 × loading buffer at 95 °C for 10 min and stored at − 20 °C. Twenty to forty micrograms of total protein sample were separated by SDS-polyacrylamide gel electrophoresis using a Bio–Rad protein assay and transferred onto polyvinylidene fluoride (PVDF) membranes. Membranes were blocked in Tris-buffered saline/0.1% Tween buffer (TBST; 25 mM Tris–Cl, 125 mM NaCl, 0.1% Tween-20) with 5% skim milk powder at room temperature for 2 h and incubated with primary antibodies at 4 °C overnight: mouse anti-GLUT5 (Santa Cruz, sc-271055, 1:300), mouse anti-GLUT3 (Abcam, ab150299, 1:1000), mouse anti-ADAM10 (Santa Cruz, sc-28358, 1:500), mouse anti-SYK (Santa Cruz, sc-1240, 1:300), rabbit anti-phospho-SYK (Tyr525/526) (CST, 2710, 1:300), mouse anti-β-tubulin and mouse anti-β-actin (Abmart, 1:1000). The corresponding secondary antibody was incubated at room temperature for 2 h (1:4000). The results were analyzed using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific, USA).

Zhang S.S., Zhu L., Peng Y., Zhang L., Chao F.L., Jiang L., Xiao Q., Liang X., Tang J., Yang H., He Q., Guo Y.J., Zhou C.N, & Tang Y. (2022). Long-term running exercise improves cognitive function and promotes microglial glucose metabolism and morphological plasticity in the hippocampus of APP/PS1 mice. Journal of Neuroinflammation, 19, 34.

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