Gallic acid, Folin-Ciocalteau reagent (2N), methanol, and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium carbonate was obtained from PanReac Quimica S.L.U. (Castellar del Vallés, Barcelona, Spain). Tryptone soy broth, tryptone soy agar, phosphate-buffered saline, and peptone water were purchased from Scharlab (Barcelona, Spain). Strains of L. innocua (CECT 910) and Escherichia coli (CECT 101) were purchased from the Spanish Type Collection (CECT, University of Valencia, Valencia, Spain).
Tryptone soy broth (tsb)
Manufactured by Scharlab
Sourced in Spain
Tryptone Soy Broth is a general-purpose microbiological culture medium used for the growth and cultivation of a wide range of bacterial species. It provides a nutrient-rich environment that supports the growth of both aerobic and anaerobic bacteria. The broth contains tryptone, a source of nitrogen, and soy peptone, which provides additional nutrients for bacterial metabolism.
Automatically generated - may contain errors
Lab products found in correlation
Tryptone soy agar, by Scharlab (3 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Peptone water, by Scharlab (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Scharlab (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Escherichia coli cect 434, by American Type Culture Collection (1 mentions)
Peg1000, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Carvacrol, by Merck Group (1 mentions)
Mueller hinton 2 broth cation adjusted, by BD (1 mentions)
Poloxamer 188, by BASF (1 mentions)
4 protocols using tryptone soy broth (tsb)
1
Antimicrobial Activity Evaluation Protocol
2
Antimicrobial Activity of Arginine-based Compound
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Decolored and deodorized Kobayashi zein powder was purchased from CBC-Iberia (Barcelona). Ethyl-N α -dodecanoyl-L-arginate hydrochloride (C 20 H 41 N 4 O 3 Cl) was kindly provided by Vedeqsa Grupo LAMIRSA (Terrassa, Barcelona, Spain) . Ethanol, acetic acid, and acetonitrile were purchased from Scharlau (Barcelona, Spain), and trifluoroacetic acid and glycerol from Sigma (Madrid, Spain). These reagents were used without further purification. Deionized water was supplied by a Millipore Milli-Q Plus purification system (Molsheim, France).
Gram-positive bacteria, Listeria monocytogenes CECT 934 (ATCC 19114) , and Gramnegative bacteria, Escherichia coli CECT 434 (ATCC 25922), were obtained from the Spanish Type Culture Collection (CECT, Valencia, Spain) and selected for use in the antimicrobial assays because of their relevance in the food industry. The strains were stored in Tryptone Soy Broth (TSB, Scharlau, Barcelona) with 20% glycerol at -80 °C until needed.
For experimental use, the stock cultures were maintained by regular subculture on Tryptone Soy Agar (TSA) slants from Scharlau (Barcelona, Spain) at 4 °C and transferred monthly.
Prior to tests, a loop of each strain was transferred to 10 ml of TSB and incubated at 37 °C for 18 h to obtain early stationary phase cells.
Gram-positive bacteria, Listeria monocytogenes CECT 934 (ATCC 19114) , and Gramnegative bacteria, Escherichia coli CECT 434 (ATCC 25922), were obtained from the Spanish Type Culture Collection (CECT, Valencia, Spain) and selected for use in the antimicrobial assays because of their relevance in the food industry. The strains were stored in Tryptone Soy Broth (TSB, Scharlau, Barcelona) with 20% glycerol at -80 °C until needed.
For experimental use, the stock cultures were maintained by regular subculture on Tryptone Soy Agar (TSA) slants from Scharlau (Barcelona, Spain) at 4 °C and transferred monthly.
Prior to tests, a loop of each strain was transferred to 10 ml of TSB and incubated at 37 °C for 18 h to obtain early stationary phase cells.
3
Development of Antimicrobial Cassava Starch-Based Bilayer Films
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Cassava starch, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) 8% and amorphous PLA 4060D with density of 1.24 g/cm 3 , supplied respectively by Asia CO., LDT (Kalasin, Thailand), NaturePlast (Caen, France) and Natureworks (U.S.A), were used to obtain bilayer films. The plasticizer poly(ethylene glycol) with molecular weight of 1000 g/mol (PEG1000) was purchased from Sigma-Aldrich (Steinheim, Germany), whereas the glycerol was obtained from Panreac Química S.L.U. (Castellar del Vall es, Barcelona, Spain). Carvacrol (CA) and the different UV grade solvents, chloroform, methanol, ethanol, acetic acid and isooctane, were supplied by Sigma-Aldrich (Steinheim, Germany). Magnesium nitrate (Mg(NO 3 ) 2 ), used to reach 53% relative humidity (RH) during the film conditioning, was supplied by Vidra Foc S.A. (Barcelona, Spain) .
For the antimicrobial activity analysis, stock cultures of Escherichia coli (CECT 101) and Listeria innocua (CECT 910) were supplied by the Spanish Type Culture Collection (CECT, Burjassot, Spain). Tryptone Soy Broth, Agar Bacteriological and Tryptone Phosphate Water were provided by Scharlab (Barcelona, Spain).
For the antimicrobial activity analysis, stock cultures of Escherichia coli (CECT 101) and Listeria innocua (CECT 910) were supplied by the Spanish Type Culture Collection (CECT, Burjassot, Spain). Tryptone Soy Broth, Agar Bacteriological and Tryptone Phosphate Water were provided by Scharlab (Barcelona, Spain).
4
Colistin Sulfate Formulation Development
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Colistin sulfate was obtained from Zhejiang Shenghua Biok Biology Co., LTD. (China). Mueller-Hinton II broth cation adjusted (MHBCA) was from Becton Dickinson and Company (Sparks, MD, US) and Tryptone Soy Agar (TSA) and Tryptone Soy Broth (TSB) were purchased from Scharlau (Sentmenat, Barcelona, Spain). Precirol® ATO 5 was kindly provided by Gattefossé (Madrid, Spain). Poloxamer 188 and Lutrol LF68 were a kind gift from BASF (Ludwigshafen, Rhineland-Palatinate, Germany). Polysorbate and Tween 80 were purchased from Panreac Química (Castellar del Vallès, Barcelona, Spain) and Miglyol 182 N/F was provided by Sasol (Hamburg, Germany).
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