A total of 400 ng of VICs RNA was reverse transcribed to single strand cDNA using High Capacity RNA-to-cDNA Master Mix (Applied Biosystems, Foster City, CA, USA) according to manufacturer’s instruction. The cDNA was amplified with TaqMan Gene Expression Assays (Hs00167155_m1 for PAI-1, Gene Symbol: SERPINE1) containing both primers and probe on an ABI PRISMR 7900HT Fast Real-Time PCR System (Applied Biosystems). Beta-actin (Hs99999903_m1, human ACTB Endogenous Control FAM/MGB Probe, Non-Primer Limited; Applied Biosystems) was used as a housekeeping gene. The comparative threshold cycle method (R = 2−ΔΔCt) was applied to analyze the obtained data.
Abi prismr 7900ht fast real time pcr system
Manufactured by Thermo Fisher Scientific
The ABI PRISMR 7900HT Fast Real-Time PCR System is a high-throughput real-time PCR instrument designed for rapid and precise gene expression analysis. The system features a 384-well block format, advanced optics, and a fast thermal cycler, enabling efficient and accurate quantification of nucleic acid targets.
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Lab products found in correlation
2 protocols using abi prismr 7900ht fast real time pcr system
1
Quantifying PAI-1 Expression in VICs
2
NF-κB p65 Rel Gene Expression Analysis
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A total of 400 ng of RNA from VICs was reverse transcribed to single-strand cDNA (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s instruction. The cDNA was amplified with TaqMan Gene Expression Assay (Hs01042014_m1 for NF-κB p65 Rel; gene symbol: RELA) containing both primers and probe on an ABI PRISMR 7900HT Fast Real-Time PCR System (Applied Biosystems). β-Actin (Hs99999903_m1, human ACTB Endogenous Control FAM/ MGB Probe, Non-Primer Limited; Applied Biosystems) was used as a housekeeping gene. To analyse the obtained data, the comparative threshold cycle method was applied [26 (link)].
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