Protein purification kit

The extraction and detection methods for hegf employ in this study were identical to that reported previously [38 (link), 39 (link)]. His-tagged hegf protein was extracted from culture supernatant using a protein purification kit (Sangon Biotech, Shanghai). The protein electrophoresis procedure was identical to the previous publication [40 (link)]. SAM was extracted and measured using our previously established method [41 (link), 42 (link)].

Protein was isolated from lung tissues after 3 d model establishment by using the protein purification kit (Sangon, Shanghai, China). Protein levels were determined using Western blot analysis following the protocol in the lab with modification [20 , 21 (link)]. The primary antibodies (Cell Signaling Technology) against p-STAT3 (1 : 2000), p-JAK-2 (1 : 2000), STAT3 (1 : 2000), JAK-2 (1 : 2000), caspase 3 (1 : 2000), BAX (1 : 2000), Bcl-2 (1 : 2000), and β-actin (1 : 2000) were used, and the HRP-conjugated anti-rabbit/mouse IgG secondary antibody (1 : 2000, Cell Signaling Technology) was used. Intensities of the bands were measured by Image Lab software (Bio-Rad) to assess relative protein levels.