Nitric oxide kit

The vascular rings were divided into 3 groups: control group, H₂O₂ group cultured in DMEM, and H₂O₂ + quercetin group cultured in DMEM containing 200 umol/L quercetin (Abcam, UK). After 1 hour, the H₂O₂ group and H₂O₂ + quercetin group were treated with 100 umol/L H₂O₂. After 4 hours, the culture supernatant was recovered, and then, the NO content was detected by the nitric oxide (NO) kit (Solarbio, China). Another 3 groups, treated as above, after 24 h, were treated by 100 umol/L H₂O₂. Then, NO, SOD, and MDA contents were detected using the relevant kits (Solarbio, China). In the mechanical damage experiment, shear the veins by scissors to form a 2 mm incised wound. The control group did not do any treatment. The wound group was cultured in DMEM, and the wound + quercetin group was cultured in DMEM containing 200 umol/L quercetin. After 24 hours, the veins were collected, and mRMA levels of IL-6, TNFα, CCL20, PCNA, and VEGF were detected by qRT-PCR. Then, the veins were collected for H&E staining, PCNA, and VEGF immunofluorescent staining.