Instant pc kit

Manufactured by Agilent Technologies

The Instant PC kit is a self-contained, portable laboratory equipment designed for quick and reliable data analysis. It provides a compact, all-in-one solution for essential laboratory functions.

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Lab products found in correlation

Formic acid, by Thermo Fisher Scientific (6 mentions) Acetonitrile, by Thermo Fisher Scientific (4 mentions) Methanol, by Thermo Fisher Scientific (4 mentions) Deep well 1 ml 96 well plate protein lobind, by Eppendorf (2 mentions) Recombinant mouse hexb protein his tag, by MyBioSource (2 mentions) Enzyme activity assay kit for β n acetyl glucosaminidase, by Merck Group (2 mentions) Recombinant nag enzyme, by New England Biolabs (2 mentions) Tris hcl buffer, by Thermo Fisher Scientific (2 mentions) Dithiothreitol (dtt), by Thermo Fisher Scientific (2 mentions) Iodoacetamide iam, by Thermo Fisher Scientific (2 mentions) Sequencing grade modified trypsin, by Promega (2 mentions) Alcohol dehydrogenase, by Merck Group (1 mentions) Myoglobin, by Merck Group (1 mentions) Ammonium bicarbonate, by Avantor (1 mentions)

2 protocols using instant pc kit

Quantitative Proteomics Protocol

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Dithiothreitol (DTT) and iodoacetamide (IAM) were purchased from Thermo Pierce (Rockford, IL). Tris–HCl buffer and high‐performance liquid chromatography (HPLC) grade solvents, methanol, acetonitrile (ACN), formic acid (FA), were purchased from Thermo Fisher Scientific (Waltham, MA). The custom stable isotopic labeled (SIL) surrogate peptide (GILIDTSR, AQUA Ultimate grade) with 13C and 15N‐labeled arginine (R*), and nonlabeled surrogate peptide were synthesized from Thermo Fisher Scientific. Sequencing grade modified trypsin was purchased from Promega (Madison, WI). Ammonium bicarbonate and urea were purchased from JT Baker (Center Valley, PA). Deep well 1‐ml 96‐well plate (Protein LoBind) was purchased from Eppendorf (Hauppauge, NY). Recombinant mouse HEXB protein (His Tag) was obtained from MyBioSource (San Diego, CA). Recombinant proteins alcohol dehydrogenase (ADH) from yeast, carbonic anhydrase ii (CA2) from Bos Taurus, myoglobin (MB) from equine heart, phosphorylase‐b (PYGM) from rabbit muscle and enolase (ENL) from baker's yeast, and enzyme activity assay kit for β‐N‐acetyl‐glucosaminidase (NAG) were purchased from Sigma (St. Louis, MO). Recombinant NAG enzyme was from New England Biolabs (Beverly, MA). Instant PC kit was from (Agilent, formerly ProZyme, Palo Alto, CA).

Li X., An Y., Liao J., Xiao L., Swanson M., Martinez‐Fonts K., Pavon J.A., Sherer E.C., Jawa V., Wang F., Gao X., Letarte S, & Richardson D.D. (2021). Identification and characterization of a residual host cell protein hexosaminidase B associated with N‐glycan degradation during the stability study of a therapeutic recombinant monoclonal antibody product. Biotechnology Progress, 37(3), e3128.

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Quantitative Proteomics Using SIL Peptides

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Dithiothreitol (DTT) and iodoacetamide (IAM) were purchased from Thermo Pierce (Rockford, IL). Tris-HCl buffer and high-performance liquid chromatography (HPLC) grade solvents, methanol, acetonitrile (ACN), formic acid (FA), were purchased from Thermo Fisher Scientific (Waltham, MA). The custom stable isotopic labeled (SIL) surrogate peptide (GILIDTSR, AQUA Ultimate grade) with 13C and 15N labeled arginine (R*), and non-labeled surrogate peptide were synthesized from Thermo Fisher Scientific (Waltham, MA). The mAbs are from the pipeline of Merck & Co., Inc., Kenilworth, NJ, USA. Sequencing grade modified trypsin was purchased from Promega (Madison, WI). Ammonium bicarbonate and urea were purchased from JT Baker (Center Valley, PA). Deep well 1-mL 96 well plate (Protein LoBind) was purchased from Eppendorf (Hauppauge, NY). Recombinant mouse HEXB protein (His Tag) was obtained from MyBioSource (San Diego, CA). Recombinant proteins alcohol dehydrogenase (ADH) from yeast, carbonic anhydrase ii (CA2) from Bos Taurus, myoglobin (MB) from equine heart, phosphorylase-b (PYGM) from rabbit muscle and enolase (ENL) from baker's yeast, and enzyme activity assay kit for β-N-Acetyl-glucosaminidase (NAG) were purchased from Sigma (St. Louis, MO).
Recombinant NAG enzyme was from New England Biolabs (Beverly, MA). Instant PC kit was from Agilent (Palo Alto, CA).

Li X., An Y., Liao J., Xiao L., Swanson M.R., Martinez‐Fonts K., Pavon J.A., Sherer E.C., Jawa V., Gao X., Letarte S., & Richardson D.D. (2020). Identification and Characterization of a Residual Host Cell Protein Hexosaminidase B Associated with N-glycan Degradation During the Stability Study of a Therapeutic Recombinant Monoclonal Antibody Product.

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