The sterility of the nanoparticles was assessed by the Department of Microbiology at the University Hospital Virgen de la Arrixaca (Murcia, Spain). Samples of 100 µL of the suspension of nanoparticles were directly inoculated, under aseptic conditions, into BACT/ALERT PF PLUS culture bottles (Ref. 410853, BioMérieux España S.A, Madrid, Spain) and incubated at 32.5 ± 2.5 °C for 14 days while aerobic and facultative anaerobic microorganisms were looking for by using the BACT/ALERT® 3D Microbial Detection System (BioMérieux España S.A., Madrid, Spain). Negative and positive controls were included. Positive bottles were subcultured into 5% sheep’s blood agar. Samples of the grown colonies were then identified in a MALDI-TOF (Matrix Assisted Laser Desorption Ionization Time-of-Flight) mass spectrometry microbial identification system (VITEK® MS, BioMérieux España S.A, Madrid, Spain) including the VITEK® MS IVD and VITEK® MS RUO, for the clinically relevant species database and the broad research database for microorganisms, respectively.
Bact alert pf plus culture bottles
Manufactured by bioMérieux
Sourced in Spain
The BACT/ALERT PF PLUS culture bottles are a type of laboratory equipment used for blood culture testing. They are designed to facilitate the detection and identification of aerobic and anaerobic microorganisms in blood samples.
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Lab products found in correlation
2 protocols using bact alert pf plus culture bottles
1
Sterility Testing of Nanoparticles
2
Detecting Mycoplasma Contamination in NK Cells
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To detect possible contamination of NK cell products with mycoplasma, we used multiplex polymerase chain reaction (PCR) with primers described in Supplementary Table 3 according to published recommendations [11] (link).
The sterility of the cell products was tested with the BACT/ALERT automated microbial detection systems (bioMERIEUX) with BACT/ALERT PF Plus culture bottles at day 0, before 7 days of infusion and in the nal cell products.
The sterility of the cell products was tested with the BACT/ALERT automated microbial detection systems (bioMERIEUX) with BACT/ALERT PF Plus culture bottles at day 0, before 7 days of infusion and in the nal cell products.
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