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Rat igg2a

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Rat igG2a is an immunoglobulin G (IgG) subclass produced by the rat immune system. It is a type of antibody that can be used in various laboratory applications, such as immunoassays and immunohistochemistry.

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Lab products found in correlation

Rat igg2b, by Bio-Rad (1 mentions) Mouse igg1, by Beckman Coulter (1 mentions) Facscan, by BD (1 mentions) Macs buffer, by Miltenyi Biotec (1 mentions) Mouse igg2b, by BioLegend (1 mentions) Polyclonal rabbit igg, by R&D Systems (1 mentions) Rat anti cd169, by Bio-Rad (1 mentions) Dylight 488 conjugated goat anti rabbit, by Jackson ImmunoResearch (1 mentions) Rabbit anti ho 1, by Abcam (1 mentions) 3 3 diaminobenzidine (dab), by Agilent Technologies (1 mentions)

4 protocols using rat igg2a

1

Flow Cytometric Characterization of Adipose-Derived Mesenchymal Stem Cells

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The cAd-MSCs phenotypes were evaluated by performing flow cytometry. The cells were pelleted at 1500 rpm for 5 min, washed with MACS buffer (Miltenyi Biotec), and then distributed into groups according to requirement. Here we had four markers including CD34(R-Phycoerythrin conjugated, clone 1H6; BD), CD44(ALEXA FLUOR® 488-conjugated, clone YKIX337.8.7; Bio-Rad), CD45(R-Phycoerythrin conjugated clone YKIX337.8.7; Bio-Rad), and CD90(clone YKIX337.8.7; Bio-Rad). Apart from CD90, other groups were immunostained for 30 min at 4°C in the dark. CD90 requires another 30 min of incubation for secondary antibody (R-Phycoerythrin conjugated, Rabbit anti-rat, Bio-Rad). Furthermore, there were three relative isotype control antibodies that need to be prepared, including Rat igG2a (ALEXA FLUOR® 488 isotype control, Bio-Rad), Rat igG2b (R-Phycoerythrin isotype control, Bio-Rad), and Mouse igG1 (R-Phycoerythrin isotype control, Beckman Coulter), which were also incubated 30 min at 4°C in the dark. After incubation, the cells were washed and resuspended with MACS buffer. The acquisition was performed by using a FACScan (BECTON DICKINSON) with 5,000 events per sample. Lastly, data were analyzed by using FlowJo software (Tree Star).
Chen C.C., Yang S.F., Wang I.K., Hsieh S.Y., Yu J.X., Wu T.L., Huang W.J., Su M.H., Yang H.L., Chang P.C., Teng A.C., Chia-Yi C, & Liang S.L. (2022). The Long-Term Efficacy Study of Multiple Allogeneic Canine Adipose Tissue-Derived Mesenchymal Stem Cells Transplantations Combined With Surgery in Four Dogs With Lumbosacral Spinal Cord Injury. Cell Transplantation, 31, 09636897221081487.
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2

Conjunctiva Basophil Scoring in Tissue

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IHC was performed using anti–mMCP-8 (clone TUG8, BioLegend). Isotype control was performed using rat IgG2a (Bio-Rad Laboratories). The scoring of basophils in conjunctival tissues followed a similar pattern to the histology evaluation above: conjunctiva that did not show any basophils was scored as a 0; if trace amounts of basophils were present, the tissue was assigned a score of 1; when more noticeable basophils were present, the conjunctiva was scored as a 2; finally, conjunctiva tissues were scored as a 3 if basophils manifested in multifocal to coalescing clusters.
Han H., Cummings S., Shade K.T., Johnson J., Qian G., Gans J., Shankara S., Escobedo J., Zarazinski E., Bodinizzo R., Bangari D., Bryce P, & Hicks A. (2023). Cellular mechanisms and effects of IL-4 receptor blockade in experimental conjunctivitis evoked by skin inflammation. JCI Insight, 8(3), e163495.
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3

Modulation of M2-Macrophage Phenotype

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Anti‐IL‐10 (10 µg mL−1, clone JES3‐12G8, rat IgG2a, AbD Serotec, Marnes‐la‐Coquette, France), anti‐IL‐10Rα (10 µg mL−1, clone 3c.5.2b, mouse IgG1, Schering‐Plough) and anti‐PD‐L1 (20 µg mL−1, clone 29E.2A3, mouse IgG2b, Biolegend, Saint‐Cyr‐L’école, France) blocking antibodies and their respective controls were used in the suppression assay and to assess the role of IL‐10 in the modulation of surface markers on in vitro generated M2‐MΦ. Anti‐M‐CSF (20 µg mL−1, polyclonal rabbit IgG, Genzyme, Lyon, France) and anti‐TGF‐β (10 µg mL−1, pan‐specific antibody, polyclonal rabbit IgG, R&D Systems) antibodies and clinical anti‐VEGF antibody (1 µg mL−1, human IgG1, Avastin®, Roche, Bale, Switzerland) were used in SNDil‐MΦ cultures.
Ramos R.N., Rodriguez C., Hubert M., Ardin M., Treilleux I., Ries C.H., Lavergne E., Chabaud S., Colombe A., Trédan O., Guedes H.G., Laginha F., Richer W., Piaggio E., Barbuto J.A., Caux C., Ménétrier‐Caux C, & Bendriss‐Vermare N. (2020). CD163+ tumor‐associated macrophage accumulation in breast cancer patients reflects both local differentiation signals and systemic skewing of monocytes. Clinical & Translational Immunology, 9(2), e1108.
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4

Immunohistochemical Staining of Rat Tissues

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The antibodies used were rat anti‐CD169 (AbD Serotec, MCA‐884; 1:200), rabbit anti‐iba1 (Waco, 019‐19741; 1:200) and rabbit anti‐HO‐1 (Abcam, ab13243; 1:250). The secondary antibodies were biotin conj. goat anti‐rat (Amersham, RPN 1005; 1:1500), followed by Vectastain ABC reagent Vector (PK‐7100) and DAB (DakoCytomation), using the companies' recommendations. For rabbit antibodies, EnVision (DakoCytomation) was used. For double‐staining RH RedX‐conjugated goat anti‐rat (Jackson, 112‐295‐102) and Dylight 488‐conjugated goat anti‐rabbit (Jackson, 111‐485.144) were used. Specimens incubated with rat IgG2a (AbD Serotec), irrelevant rabbit antibodies or no primary antibodies served as controls.
Mikkelsen H.B., Huizinga J.D., Larsen J.O, & Kirkeby S. (2017). Ionized calcium‐binding adaptor molecule 1 positive macrophages and HO‐1 up‐regulation in intestinal muscularis resident macrophages. Anatomical Record (Hoboken, N.j. : 2007), 300(6), 1114-1122.
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