Immunohistostaining was performed following the protocol previously described7 (link) with modifications, and by using mouse monoclonal anti-lamin A/C (MABT538, clone 2E8.2, Millipore Sigma; 1:75 dilution) antibody or rabbit polyclonal anti-progerin antibody (Collins, custom; 1:75 dilution). Briefly, ascending aorta sections were dewaxed and rehydrated, and the antigens were retrieved by heating in EDTA buffer (1 mM, pH 8.0) for 2 min in a pressure cooker. Tissue sections were blocked in TBS buffer containing 10% donkey serum and 1% BSA, and then incubated with a Mouse-on-Mouse blocking reagent (Vector Laboratories) to reduce endogenous mouse antibody binding. Slides were incubated with the above primary antibody overnight at 4 °C. After washing thoroughly in TBS, the sections were then incubated with donkey anti-mouse Alexa Fluor 594-conjugated or donkey anti-rabbit Alexa Fluor 488-conjugated secondary antibodies (ThermoFisher Scientific; 1:3000 dilutions). All tissue sections were mounted in DAPI-containing medium (Vector Laboratories). Fluorescence images were captured by a confocal microscope system (Zeiss LMS 880) with 40x water lens.
Anti progerin antibody
Manufactured by Merck Group
The Anti-progerin antibody is a laboratory tool used for the detection and analysis of progerin, a protein associated with premature aging conditions. It provides a specific and reliable method for researchers to identify and quantify progerin in various biological samples.
Automatically generated - may contain errors
2 protocols using anti progerin antibody
1
Immunohistostaining of Aortic Lamins
2
Immunohistostaining of Aortic Lamins
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Immunohistostaining was performed following the protocol previously described7 (link) with modifications, and by using mouse monoclonal anti-lamin A/C (MABT538, clone 2E8.2, Millipore Sigma; 1:75 dilution) antibody or rabbit polyclonal anti-progerin antibody (Collins, custom; 1:75 dilution). Briefly, ascending aorta sections were dewaxed and rehydrated, and the antigens were retrieved by heating in EDTA buffer (1 mM, pH 8.0) for 2 min in a pressure cooker. Tissue sections were blocked in TBS buffer containing 10% donkey serum and 1% BSA, and then incubated with a Mouse-on-Mouse blocking reagent (Vector Laboratories) to reduce endogenous mouse antibody binding. Slides were incubated with the above primary antibody overnight at 4 °C. After washing thoroughly in TBS, the sections were then incubated with donkey anti-mouse Alexa Fluor 594-conjugated or donkey anti-rabbit Alexa Fluor 488-conjugated secondary antibodies (ThermoFisher Scientific; 1:3000 dilutions). All tissue sections were mounted in DAPI-containing medium (Vector Laboratories). Fluorescence images were captured by a confocal microscope system (Zeiss LMS 880) with 40x water lens.
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