Madin-Darby Canine Kidney (MDCK) epithelial cells and Ras V12 , a stable MDCK cell line expressing GFP-Ras V12 , in a tetracycline-inducible manner were cultured in DMEM with 10% fetal bovine serum (FBS), 50mg/ml streptomycin and 50U/ml penicillin antibiotics (all from Hyclone Laboratories Inc.). The Ras V12 line was a kind gift of Yasuyuki Fujita (Hokkaido Univ) described here 25 . Cells were cultured at 37°C in a 5% CO2 incubator on 100mm tissue culture dishes (Corning). Samples were either grown in mono-culture or in co-culture with a ratio of 1:10 Ras V12 to wild type (WT) MDCK cells to create monolayers. The Ras V12 cells were activated with tetracycline at a concentration of 2μg/ml and experiments were performed 1 day later. The inhibitor Y-27632 was used at a concentration of 10μM in a 1 day treatment.
Penicillin antibiotics
Manufactured by Cytiva
Penicillin antibiotics are a class of medications used to treat bacterial infections. They work by inhibiting the synthesis of the bacterial cell wall, which is essential for the survival and growth of bacteria. Penicillin antibiotics are commonly used to treat a variety of bacterial infections, such as pneumonia, skin infections, and urinary tract infections.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Cytiva (6 mentions)
100mm tissue culture dishes, by Thermo Fisher Scientific (5 mentions)
Dulbecco modified eagle medium (dmem), by Cytiva (5 mentions)
100 mm tissue culture dishes, by Corning (1 mentions)
Dulbecco s modified, by Cytiva (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Cytiva (1 mentions)
6 protocols using penicillin antibiotics
1
Ras V12 MDCK Cell Co-Culture Protocol
2
Culturing NIH3T3 Fibroblast Cells
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NIH3T3 fibroblast (ATCC) cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) (Hyclone Laboratories, Inc.) supplemented with 10% fetal bovine serum (FBS), 50 mg/ml streptomycin, and 50 U/ml penicillin antibiotics (all from Hyclone Laboratories Inc.). Cells were grown at 37°C with 5% CO2 on 100 mm tissue culture dishes (Fisher) until 80%–90% confluent.
3
Culturing Fibroblast and Muscle Cells
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NIH3T3 fibroblast (ATCC) and C2C12 skeletal muscle (ATCC) cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Hyclone Laboratories Inc.). Human ASM cells (Donor 12) (previously characterized by Gosens et al.80 ) immortalized by stable transfection with human telomerase reverse transcriptase were obtained as a generous gift from Dr. William Gerthoffer (University of South Alabama) and maintained in DMEM/F12 (Invitrogen 11330). All culture media was supplemented with 10% fetal bovine serum (FBS), 100 mg/ml streptomycin and 100U/ml penicillin antibiotics (all from Hyclone Laboratories Inc.). Prior to microtissue seeding, cells were grown at 37 °C with 5% CO2 on 100 mm tissue culture dishes (Fisher) until 80–90% confluent.
4
Culturing NIH3T3 Fibroblasts for Microtissue
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Prior to microtissue fabrication, NIH3T3 (ATCC) fibroblast cells were maintained on 100mm tissue culture dishes (Fisher) at 37℃ with 5% CO 2 until 80--90% confluent in feeder media composed of Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100mg/ml streptomycin and 100U/ml penicillin antibiotics (all from Hyclone Laboratories Inc.).
5
Culturing NIH3T3 Fibroblasts in DMEM
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NIH3T3 fibroblast (ATCC) cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) (Hyclone Laboratories Inc.) supplemented with 10% fetal bovine serum (FBS), 50mg/ml streptomycin and 50U/ml penicillin antibiotics (all from Hyclone Laboratories Inc.). Cells were grown at 37℃ with 5% CO 2 on 100mm tissue culture dishes (Fisher) until 80-90% confluent.
6
Culturing NIH3T3 Fibroblasts for Microtissue
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Prior to microtissue fabrication, NIH3T3 (ATCC) fibroblast cells were maintained on 100mm tissue culture dishes (Fisher) at 37℃ with 5% CO 2 until 80--90% confluent in feeder media composed of Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100mg/ml streptomycin and 100U/ml penicillin antibiotics (all from Hyclone Laboratories Inc.).
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