7890a gc system supelcowax 10 capillary gc column

Gas chromatography was performed using the Agilent Technologies 7890A GC System (SUPELCOWAX™ 10) Capillary GC Column (15 mm × 0.10 mm, 0.10 μm, Supelco, Bellefonte, PA, USA). Chromatographic conditions were as follows: the initial temperature was 40 °C for 0.5 min, and then it increased at a rate of 25 °C/min to 195 °C (0 min). Next, it was increased at a rate of 3 °C/min to 205 °C (0 min), and then increased at a rate of 8 °C/min to 250 °C for 0.5 min. The total analysis lasted approximately 16.16 min and the gas flow rate was 1 mL/min with hydrogen as the carrier gas. Fatty acids were identified by comparing their retention times with those of commercially available standards. The analysis of fatty acid contents in particular samples was performed using specialist ChemStation Software (Agilent Technologies Inc, London, UK). The qualitative identification of fatty acids was performed by comparing retention times to those of the standards, and the quantity of a specific fatty acid was given as a percentage of the fatty acid in the total amount of analyzed compounds (excluding the standard, heneicosanoic acid (C21:0)).

Fatty acids methyl esters were isolated from serum with the use of the modified Folch and Szczuko methods [88 (link),89 (link)]. The fatty acids profile was labelled by gas chromatography. The gas chromatography (GC) was performed with the use of the Agilent Technologies 7890A GC System (SUPELCOWAX™)10 Capillary GC Column (15 mm × 0.10 mm, 0.10 μm); Supelco (Bellefonte, PA, USA). Chloroform and methanol (Merck), boron trifluoride in methanol, NaCl and 2,6-Di-tert-butyl-4-methylphenol (Sigma-Aldrich, St Louis, MO, USA), double-distilled water (Milli-Q Water System, Millipore, Billerica, MA, USA), fatty acids standards were supplied by Sigma-Aldrich (Darmstadt, Germany), Cayman Chemical (Ann Arbor, MI, USA) and Neochema (Bodenheim, Germany). The results of the fatty acids were presented as the percentage distribution.