Dulbecco s modified eagle s medium dmem f12

BJ (ATCC-CRL 2522) and SCC-15 (ATCC-CRL-1623) cell lines and Dulbecco’s Modified Eagle’s Medium (DMEM-F12), Eagle’s minimum essential medium (EMEM), fetal bovine serum (FBS), penicillin, streptomycin, trypsin/ethylenediaminetetraacetic acid solution, and phosphate-buffered saline (PBS; with and without magnesium and calcium ions) were obtained from American Type Culture Collection (ATCC) (Manassas, VA, USA). Hydrocortisone, Trypan blue, and fluorescein isothiocyanate (FITC) were provided by Sigma-Aldrich Co. (St Louis, MO, USA). The fluorescent markers 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI), MitoTracker^® Deep Red FM and Rhodamine Red™-X (succinimidyl ester, 5-isomer), ATP Determination Kit, and CyQUANT^® NF Cell Proliferation Assay Kit were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Cell culture sterile plastics were purchased from Corning Incorporated (Corning, NY, USA), Sigma-Aldrich Co., and Brand GMBH + CO KG (Wertheim, Germany).