MDA-MB-231-GFP treated cells were trypsinized with 0.25% trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA) for 2 minutes at 37 °C, quenched with DMEM supplemented with 10% FBS, and pelleted by centrifugation at 800xg for 2 minutes. The cell pellet was resuspended with 1xPBS supplemented with 1 % FBS into a 12×75mm test tube with a cell strainer cap (Falcon, Durham, NC, USA) and analyzed with the DxP FACScan (Cytek Biosciences, Fremont, CA, USA). The data was analyzed using FCS Express 5 (De Novo Software, Glendale, CA, USA).
Dxp facscan
Manufactured by Cytek
Sourced in United States
The DxP FACScan is a flow cytometry instrument manufactured by Cytek. It is designed to analyze and sort cells based on their physical and fluorescent characteristics. The DxP FACScan utilizes multiple laser excitation sources and detectors to provide high-resolution data on various cellular parameters.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin edta, by Thermo Fisher Scientific (3 mentions)
Cell strainer cap, by BD (1 mentions)
Fcs express 5, by De Novo Software (1 mentions)
Human fc block, by BD (1 mentions)
Cd123 pe cy5, by BD (1 mentions)
Aqua live dead stain, by Thermo Fisher Scientific (1 mentions)
Rbs lysis buffer, by BioLegend (1 mentions)
Cd19 pe, by BD (1 mentions)
Hla dr pe cy7, by BioLegend (1 mentions)
2 protocols using dxp facscan
1
GFP-labeled MDA-MB-231 Cell Analysis
2
Multiparametric Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After washing, treated cells and controls were incubated with human Fc-block (BD), and stained with the following antibodies as described in figure legends (at 1:100 dilution unless stated): IgE-FITC (eBiosciences clone: IgE21), FcɛRIα-APC (eBiosciences clone AER-37 at 1:50 dilution), anti-biotin AF-488 (eBiosciences clone: BK-1/39), anti-mouse-lambda-light-chain PE (BioLegend clone: RML-42), CD123 PE-Cy5 (BD clone: 9F5 at 1:20 dilution), HLA-DR PE-Cy7 (BioLegend clone L243 at 1:80 dilution), CD203c BV421 (BioLegend clone: NP4D6 at 1:50 dilution), CD19-PE (BD clone: HIB19 at 1:50), CD23-BV421 (BioLegend EBVCS-5 at 1:50 dilution) and Aqua Live Dead stain (Life Technologies). Stained cells were then lysed with RBS lysis buffer (BioLegend) for 5 min at room temperature, and washed with FACS buffer (PBS pH 7.4 supplemented with 10% FCS). Data were collected on a DxP FACSCAN from Cytek Development in Fremont, CA (10 colours with three lasers—488, 639, 407) using FlowJo CE and analysed using FlowJo (version 10).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!