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Sodium salt of n hydroxysulfosuccinimide s nhs

Manufactured by Chem-Impex
Sourced in Germany

Sodium salt of N-hydroxysulfosuccinimide (s-NHS) is a water-soluble, amine-reactive crosslinking agent used in various biochemical applications. It acts as a coupling reagent to facilitate the formation of amide bonds between carboxyl groups and primary amine groups.

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2 protocols using sodium salt of n hydroxysulfosuccinimide s nhs

1

Biomolecular Coupling Protocol

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Inorganic salts and tris(hydroxymethyl)aminomethane (tris) were purchased from MP Biomedicals (Illkirch, France) and Sigma-Aldrich (St. Louis, MO, USA). Human thrombin with a specific activity of 4.2 kIU/mg and human factor Xa were purchased from Haematologic Technologies (Essex Junction, VT, USA). Ethanolamine from Sigma-Aldrich (St. Louis, MO, USA), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC-HCl) from Roth (Karlsruhe, Germany), and sodium salt of N-hydroxysulfosuccinimide (s-NHS) from Chem-Impex Int'l (Wood Dale, IL, USA) were used. DNA oligonucleotides were synthesized and purified by polyacrylamide gel electrophoresis by Synthol (Moscow, Russia). Ultra-pure water (18.2 MΩ•cm) was obtained with Milli-Q (Merck KGaA, Darmstadt, Germany) and used to prepare all buffers and solutions.
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2

Preparation and Characterization of BoNT-A Solution

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Inorganic salts, sucrose, Tween 80, human serum albumin, and gamma globulin (IgGHum) (AppliChem GmbH, Darmstadt, Germany and Sigma–Aldrich, St. Louis, MO, USA) were used in this work. A commercial set of bovine blood serum samples was used (Biolot, Saint Petersburg, Russia).
All experiments were performed in ultrapure water prepared with Milli-Q equipment (Merck KGaA, Darmstadt, Germany). PBS was prepared from tablets from EcoService (Saint Petersburg, Russia). HEPES buffer, pH 7.5, from AppliChem (Darmstadt, Germany), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC-HCl) from Roth (Karlsruhe, Germany), and sodium salt of N-hydroxysulfosuccinimide (s-NHS) from Chem-Impex Int’l (Wood Dale, IL, USA) were used.
Xeomin 50 Units per vial (Merz therapeutics, Frankfurt am Main, Germany) contains BoNT serotype A in human serum albumin with sucrose. The powder was dissolved in 125 µL Ringer’s solution (Grotex, Saint Petersburg, Russia) providing a solution with 400 Units/mL BoNT, 8 mg/mL human serum albumin, and 38 mg/mL sucrose. The solution was exploited immediately after the preparation. According to Frevert [21 (link)], 400 Units/mL of Xeomin corresponds to 1.76 ng/mL or 11.7 pM of BoNT serotype A.
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