UPEC strain CFT073 (ATCC # 700928; United States), Commensal strain K12 MG1655 (ATCC # 700926; United States), Luria–bertani broth (Himedia cat # M1245; India), Agar (Himedia cat # GRM666; India), C. elegans Bristol wild type N2 strain , E.coli OP50, THP-1 cells (ATCC # TIB-202; United States), RPMI-1640 (Gibco Cat# 11875119; United States), Hela cells (ATCC # CCL2; United States), DMEM (Cat#L0104, Biowest, Nuaille, France), fetal bovine serum (Gibco Cat# 10270106; United States), T24 cells (ATCC# HTB-4™; United States), McCoy’s 5A modified Media(Gibco Cat# 16600082),3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide-MTT (Invitrogen Cat # M6494; United States), cas9 Protein (Invitrogen Cat # A36497; United States), 1-Ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) (Himedia Cat# RM1817, India), N-hydroxysulfosuccinimide (Sulfo-NHS) (Himedia Cat# RM1120, India), HEPES buffer solution (Sigma-Aldrich Cat # 83264; United States), GeneArt™ Precision gRNA Synthesis Kit (Invitrogen Cat # A29377, United States), TRI Reagent (Sigma Cat # T9424; United States), RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific Cat # K1622; United States), SYBR Green Master Mix (Appliedbiosystems Cat# A25742; United States), Crystal violet ( SRL Cat# 28376, India) and SYTO9 green fluorescent nucleic acid stain (Invitrogen Cat # S34854, United States) were used.
T24 cells
Manufactured by American Type Culture Collection
Sourced in United States
T24 cells are a human bladder cancer cell line derived from a transitional cell carcinoma. They are adherent cells and can be used for various cell-based assays and research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (2 mentions)
Raw264.7 macrophage, by American Type Culture Collection (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
5637 cells, by American Type Culture Collection (2 mentions)
Sv huc 1 cells, by American Type Culture Collection (2 mentions)
Geneart precision grna synthesis kit, by Thermo Fisher Scientific (1 mentions)
Hepes buffer solution, by Merck Group (1 mentions)
Mccoy s 5a modified media, by Thermo Fisher Scientific (1 mentions)
Hela cell, by American Type Culture Collection (1 mentions)
Syto 9 green fluorescent nucleic acid stain, by Thermo Fisher Scientific (1 mentions)
Cas9 protein, by Thermo Fisher Scientific (1 mentions)
Sybr green master mix, by Thermo Fisher Scientific (1 mentions)
Tri reagent, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Biowest (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Sc75741, by Selleck Chemicals (1 mentions)
Igg2a fitc, by BD (1 mentions)
Minimum essential medium (mem), by Merck Group (1 mentions)
17 protocols using t24 cells
1
Comparative Analysis of Pathogenic and Commensal E. coli
2
Investigating Inflammatory Response in Bladder Cancer
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RAW 264.7 macrophages and T24 cells were purchased from the ATCC (Rockville, MD, USA). Antibodies for western blotting were purchased from Cell Signaling Technologies (Pickering, ON, CAN), including those targeting P65, P-P65, TAK1, iKKa/b, INOS, COX2, IKB, p-IKB, NLRP3, Caspase-1, and GAPDH. Secondary antibodies for western blotting and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) were purchased from Sigma (Sigma Aldrich, St. Louis, MO, USA). Transitional cell carcinoma of the bladder cells (T24 cells) and a mouse macrophage cell line (RAW 264.7 macrophages) were purchased from the American Type Culture Collection (Rockville, MD, USA). Fetal bovine serum (FBS), Dulbecco's modified Eagle's medium (DMEM) and penicillin/streptomycin solution were purchased from HyClone (Logan, UT, USA). Phycoerythrin (PE)-conjugated anti-CD80 and fluorescein isothiocyanate (FITC)-conjugated anti-mouse CD282, PE-IgG2a and FITC-IgG2a antibodies, were purchased from BD Systems (BD Biosciences, USA). ProcartaPlexTM Multiplex Immunoassay Kits were purchased from eBioscience (San Diego, CA, USA). DEAE-52 and Sephadex G-100 gel filtration medium were purchased from GE Healthcare Bio-Sciences AB (UPPPala, Sweden). SC75741 (purity, 99.79%) was purchased from Selleck (Shanghai, China).
3
Culturing EGFR-expressing Bladder Carcinoma Cells
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The T24 cells (EGFR-expressing human bladder carcinoma cell line; ATCC, Rockville, MD, USA) were cultured in McCoy’s 5a medium supplemented with 10% FBS, 1.5 mM l -glutamine, and 100 U mL−1 penicillin-streptomycin, and were maintained in a 37 °C incubator that was balanced with 5% CO2 and 100% humidity.
4
Culturing Human Bladder Cancer T24 Cells
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Human bladder cancer T24 cells (ATCC, Manassas, VA, USA) were cultured with a proliferation medium composed of Eagle’s Minimum Essential Medium (MEM) (Sigma-Aldrich, Saint Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS), 1% L-glutamine, 0.1-mM nonessential amino acids, and 1% penicillin/streptomycin (Sigma-Aldrich). Cells were grown at 37 °C in a humified atmosphere with 5% CO2.
5
Culturing T24 Bladder Cancer Cells
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T24 cells were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA) and then were maintained by Dulbecco’s modified Eagle medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. T24 cells were incubated in a humidified incubator at 37 ℃ containing 5% CO2. Before the experiment, cell density was quantified by a hemocytometer.
6
Authenticated Bladder Cell Lines for Research
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Twelve bladder cell lines, consisting of 10 urothelial carcinoma, 1 papilloma (RT4) and 1 benign bladder cell line (UROtsa), were available for the prescribed studies. Cell lines were obtained from the Pathology Core of the Bladder Cancer SPORE at MD Anderson Cancer Center (RT112, RT4, 5637, UM-UC-1, UM-UC-3, UM-UC-13, UM-UC-14, TCCSUP, 253-J, and 253J-BV cells), T24 cells from American Type Culture Collection (Manassas, VA, USA), and the UROtsa benign human bladder cell line from Dr. Donald Sens of the University of North Dakota School of Medicine, Grand Forks, ND. Prior to experimentation, the cell lines were authenticated by the Genetic Resources Core Facility at Johns Hopkins (Baltimore, MD, USA).
7
Apoptosis Analysis of T24 Cells
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Human urinary bladder transitional cell carcinoma T24 cells were obtained from American Type Culture Collection (Manassas, VA, USA). The cells were maintained in RPMI 1640 medium (WelGENE Inc., Daegu, Republic of Korea), supplemented with 10% Apoptosis Detection Kit (BD Biosciences, Franklin Lakes, NJ, USA) was used as described previously (35) . Briefly, both attached and floating cells were collected and then washed twice with ice old PBS (resuspended in 500 μL binding buffer). The cells were stained with FITC-conjugated annexin V and propidium iodide (PI) at room temperature for 15 min in the dark. Subsequently, the cells were analyzed using a flow cytometer (Becton Dickinson, San Jose, CA, USA) according to the manufacturer's protocol.
8
T24 Cell Culture with Cordycepin
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T24 cells were purchased from the American Type Culture Collection (Manassas, MD, USA). Cells were cultured at 37°C in 5% CO 2 humidified incubator in complete media consisting of RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin (WelGENE Inc., Daegu, Republic of Korea). Cordycepin obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA) was dissolved in dimethylsulfoxide (DMSO, Sigma-Aldrich Chemical Co.) to a final concentration of 100 μg/mL, and prior to use, the stock solution was diluted with cell culture medium to the desired concentration.
9
Culturing Normal and Bladder Cancer Cells
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Normal human urothelial cells (NHU) were kindly provided by Dr. Jennifer Southgate (University of York, UK) and cultured as previously described (Wezel et al., 2013 (link)). Primary bladder cancer-derived cell lines including T24 cells (human muscle invasive bladder cancer cell line HTB-4) and 5637 cells (human non-muscle invasive bladder cancer cell line HTB-9) were purchased from the American Type Culture Collection (ATCC, Manassas, VA) and cultured in DMEM (GIBCO, Waltham, MA) supplied with 10% FBS.
10
Culturing Human Bladder Cancer T24 Cells
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Human bladder cancer T24 cells used in this study were purchased from American Type Culture Collection (ATCC). ATCC-formulated McCoy’s 5A modified medium with 10% fetal bovine serum and 0.1% penicillin-streptomycin was used to culture the cells at 37 °C and 5% CO2. The cells were passaged every 3 to 4 d using 0.05% Trypsin-EDTA (Gibco).
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