Akita mice

All procedures were carried out in accordance with relevant guidelines and regulations as approved by the Animal Care Committees of The University of Tokyo and the Institute of Medical Science, Asahi Life Foundation, and complied with the standards stated in the “Guide for the Care and Use of Laboratory Animals” (National Institutes of Health, revised 2011). The study is reported in accordance with ARRIVE guidelines. Male mice were 6–55 weeks of age at the time of the study. They were housed in cages and maintained on a 12 h light–dark cycle with access to chow and water ad libitum. In these experiments, we used normal chow diet consisting of 24.9% (wt/wt) proteins, 4.6% fibers, 7.1% ashes, 49.5% carbohydrates, 4.8% fat and 9.1% water (CE-2, CLEA Japan Inc.) or high-fat diet consisting of 25.5% (wt/wt) protein, 2.9% fibers, 4.0% ashes, 29.4% carbohydrates, 32.0% fat and 6.2% water (High Fat Diet 32, CLEA Japan Inc.). High-fat diet was fed to male mice from 6 weeks old onwards. C57Bl/6 mice were purchased from Charles River Laboratories Japan, Inc. and Japan SLC, Inc. Akita mice were purchased from Japan SLC, Inc.