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Cann cg foxn1 crlcrlj nu nu

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The CAnN.Cg-Foxn1/CrlCrlj nu/nu is a laboratory mouse strain that is athymic, or lacking a functional thymus gland. This strain is commonly used in research involving xenografts and immunodeficiency studies.

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Lab products found in correlation

Bright glo luciferase assay system, by Promega (1 mentions) Cell lysis buffer, by Cell Signaling Technology (1 mentions) Envision spectrophotometer, by PerkinElmer (1 mentions) Vivoglo luciferin, by Promega (1 mentions) Balb c nu nu, by Charles River Laboratories (1 mentions) C b 17 icr scid scidjcl, by CLEA Japan (1 mentions)

Spelling variants of this product

BALB/c-nu/nu mice (CAnN. Cg-Foxn1/CrlCrlj nu/nu) (2 citations)

5 protocols using cann cg foxn1 crlcrlj nu nu

1

Evaluating Tumor Growth Inhibition In Vivo

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To evaluate tumor growth inhibition in vivo, SJCRH30-luc cells (3 × 106 in HBSS) were implanted s.c. into the right flank of nude mice (CAnN.Cg-Foxn1/CrlCrlj nu/nu; Charles River Japan). Tumor volume (TV) was calculated using the formula: TV = ab2/2, where a and b represent tumor length and width, respectively. Once the tumors had reached a volume of approximately 100 mm3, animals were randomly assigned to two groups (n = 5) and treatment was initiated. Twice-weekly i.v. injection of CF172 (25 mg/kg per injection) was carried out for 14 days.
For the detection of lymph node metastasis, the resected lymph nodes were sonicated and lysed with 100 μL Cell Lysis Buffer (Cell Signaling Technology, Beverly, MA, USA) containing a complete protease inhibitor cocktail (Roche Diagnostics). The same amount of Bright-Glo luciferase assay system (Promega) was added. Assays were quantified by reading luminescence using an EnVision spectrophotometer (PerkinElmer).
Kashima K., Watanabe M., Sato Y., Hata J., Ishii N, & Aoki Y. (2014). Inhibition of metastasis of rhabdomyosarcoma by a novel neutralizing antibody to CXC chemokine receptor-4. Cancer Science, 105(10), 1343-1350.
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2

BALB-nu/nu Mice Experimental Protocol

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Five-week-old male BALB-nu/nu mice (CAnN.Cg-Foxn1/CrlCrlj nu/nu) were obtained from Charles River Laboratories Japan (Yokohama, Japan). All animals were housed in a pathogen-free environment under controlled conditions (temperature 20–26 °C, humidity 40–70 %, light:dark cycle 12/12 h). Chlorinated water and irradiated food were provided ad libitum. All animals were allowed to acclimatize and recover from shipping-related stress for 1 week before the study. The health of the mice was monitored by daily observation. All animal experiments were conducted in accordance with the Guidelines for the Accommodation and Care of Laboratory Animals promulgated in Chugai Pharmaceutical Co., Ltd.
Yamashita-Kashima Y., Shu S., Yorozu K., Hashizume K., Moriya Y., Fujimoto-Ouchi K, & Harada N. (2014). Importance of formalin fixing conditions for HER2 testing in gastric cancer: immunohistochemical staining and fluorescence in situ hybridization. Gastric Cancer, 17(4), 638-647.
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3

Xenograft Tumor Monitoring in Mice

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SJCRH30-luc cells (2 × 106 in HBSS) were i.p. injected into nude mice (CAnN.Cg-Foxn1/CrlCrlj nu/nu; Charles River Japan, Yokohama, Japan). On the same day of SJCRH30-luc inoculation, 25 mg/kg CF172 was i.v. injected in a single shot. On day 5 after i.p. or intraperitoneal administration of luciferase substrate (65 mg/kg VivoGlo Luciferin; Promega, Madison, WI, USA), the anesthetized mice were imaged using an in vivo imaging system (NightOWL L983; Berthold Technologies, Bad Wildbad, Germany). Luciferase signals were visualized and quantified using IndiGO version 2.0.0.22 (Berthold Technologies) imaging software for NightOWL.
Kashima K., Watanabe M., Sato Y., Hata J., Ishii N, & Aoki Y. (2014). Inhibition of metastasis of rhabdomyosarcoma by a novel neutralizing antibody to CXC chemokine receptor-4. Cancer Science, 105(10), 1343-1350.
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4

Xenograft Mouse Model Development

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All animal experiments were reviewed and approved by the Institutional Animal Care and Use Committee at Chugai Pharmaceutical Co., Ltd., which is an institute accredited by AAALAC International and conformed to the Guide for the Care and Use of Laboratory Animals published by the Institute for Laboratory Animal Research. Male 5-week-old SCID mice (C.B-17/Icr-scid/scidJcl) were purchased from CLEA Japan, Inc (Tokyo, Japan). Male 4–5-week-old BALB/c-nu/nu mice (CAnN Cg-Foxn1 /CrlCrlj nu/nu) were purchased from Charles River Laboratories Japan, Inc (Yokohama, Japan). All animals were acclimatized for > 5 days prior to the study. Chlorinated water and irradiated food were provided ad libitum, and the animals were kept in a controlled 12/12 h light-dark cycle.
Furugaki K., Fujimura T., Mizuta H., Yoshimoto T., Asakawa T., Yoshimura Y, & Yoshiura S. (2023). FGFR blockade inhibits targeted therapy-tolerant persister in basal FGFR1- and FGF2-high cancers with driver oncogenes. NPJ Precision Oncology, 7, 107.
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5

BALB-nu/nu Mouse Acclimation and Care

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Female, 5-week-old BALB-nu/nu mice (CAnN.Cg-Foxn1/CrlCrlj nu/nu) were obtained from Charles River Laboratories Japan, Inc. (Yokohama, Japan). All animals were allowed to acclimatize and recover from shipping-related stress for 1 week prior to the study. The health of the mice was monitored by daily observation. The animals were allowed free access to chlorinated water and irradiated food, and the animals were kept under a controlled light-dark cycle (12–12 h). All animal experiments were reviewed and approved by the Institutional Animal Care and Use Committee at Chugai Pharmaceutical Co. Ltd.
Yamashita-Kashima Y., Shu S., Yorozu K., Moriya Y, & Harada N. (2017). Mode of action of pertuzumab in combination with trastuzumab plus docetaxel therapy in a HER2-positive breast cancer xenograft model. Oncology Letters, 14(4), 4197-4205.
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