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Anti cd56 fitc ncam16.2

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Anti-CD56-FITC (NCAM16.2) is a fluorescently-labeled monoclonal antibody that binds to the CD56 (neural cell adhesion molecule, NCAM) antigen. CD56 is expressed on natural killer cells, a subset of T cells, and some other cell types.

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Lab products found in correlation

Anti cd11c pecy7, by Thermo Fisher Scientific (1 mentions) Facsaria, by BD (1 mentions) Anti cd3 fitc sk7, by BD (1 mentions) Macs separation buffer, by Miltenyi Biotec (1 mentions) Anti cd20 fitc, by BD (1 mentions) Anti cd8 bv786 rpa t8, by BD (1 mentions)

Spelling variants of this product

CD56-FITC (41 citations) Anti-CD56 (39 citations) Anti-CD56-FITC (19 citations) NCAM16.2 (8 citations) CD56 (NCAM16.2; (5 citations) Anti‐CD56(NCAM16.2) (3 citations)

2 protocols using anti cd56 fitc ncam16.2

1

Profiling Inflammatory Cells in Rheumatoid Arthritis

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Synovial fluid was collected from inflamed joints of 6 RA patients (active disease according to the American College of Rheumatology criteria, non-biologicals treated). From these samples, cells were collected by centrifugation and either stored in liquid nitrogen or used directly. Cells were stained using anti-CD3-FITC (SK7; 1:50; BD Biosciences), anti-CD19-FITC (HD37; 1:50; Dako), anti-CD20-FITC (L27; 1:50; BD Biosciences), anti-CD56-FITC (NCAM16.2; 1:50; BD Biosciences), anti-CD66b-FITC (CLB-B13.9; 1:50; Sanquin), anti-CD11c-PECy7 (3.9; 1:200; eBioscience), anti-CD14-phycoerythrin (PE; MϕP9; 1:100; BD Biosciences) and anti-CD163-allophycocyanin (GHI/61; 1:150; BioLegend) and FACS (fluorescence-activated cell sorting) sorted (FACSAria, BD Biosciences). CD3−CD19−CD20−CD56−CD66b−CD11c+CD14+CD163+ cells were stimulated as indicated.
Vogelpoel L.T., Hansen I.S., Rispens T., Muller F.J., van Capel T.M., Turina M.C., Vos J.B., Baeten D.L., Kapsenberg M.L., de Jong E.C, & den Dunnen J. (2014). Fc gamma receptor-TLR cross-talk elicits pro-inflammatory cytokine production by human M2 macrophages. Nature Communications, 5, 5444.
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2

Multi-parameter Flow Cytometric Profiling of MDSC Subsets

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Cells (1×106 per tube) were Fc blocked for 5 min, using 2 μl anti-CD32 (catalog no. 555447; BD Biosciences), and the following antibodies were added in magnetic-activated cell sorting (MACS) separation buffer (Miltenyi Biotec, Inc., Auburn, CA, USA): Anti-HLA-DR-phycoerythrin (PE; G46-6; catalog no. 555812), anti-CD14-PacificBlue (M5E2; catalog no. 558121), anti-CD15-allophycocyanin (APC; HI98; catalog no. 551376), anti-CD3-fluorescein isothiocyanate (FITC; SK7; catalog no. 349201), anti-CD56-FITC (NCAM16.2; catalog no. 340410), anti-CD20-FITC (L27; catalog no. 347673), anti-CD19-FITC (SJ25C1; catalog no. 340719), anti-CD4-Paci-ficBlue (RPA-T4; catalog no. 561844) and anti-CD8-BV786 (RPA-T8; catalog no. 563823), purchased from BD Biosciences. Antibodies were incubated for 30 min at 4°C at concentrations indicated by the manufacturer. Cells were subsequently fixed with 1% paraformaldehyde in MACS separation buffer, and samples were analyzed on an LSR II (BD Biosciences). One aliquoted and cryopreserved healthy control blood sample served as an interexperimental control in serial flow cytometric analyses. Granulocytic MDSCs were defined as HLA-DRlo/−CD15+, and monocytic MDSCs as HLA-DRlo/−CD14+, as described previously (29 (link)).
Ilkovitch D, & Ferris L.K. (2016). Myeloid-derived suppressor cells are elevated in patients with psoriasis and produce various molecules. Molecular Medicine Reports, 14(4), 3935-3940.
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